Developmental regulation of genomic imprinting during gametogenesis.
Developmental regulation of genomic imprinting during gametogenesis.
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DOI：
10.1006/dbio.1995.0021
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发表时间：
1995-11
影响因子：
2.7
通讯作者：
A. Villar;E. M. Eddy;R. Pedersen
中科院分区：
文献类型：
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作者：
A. Villar;E. M. Eddy;R. Pedersen
哺乳动物的成功发育需要雄性和雌性基因组。这在一定程度上是由于基因组印记，这导致后代只能从母亲或父亲那里继承一个功能性基因拷贝。有证据表明，这种父母基因组的专门化是在配子发生过程中建立的，当时从父母那里继承的印记模式被转换以反映后代的性别。我们使用逆转录-PCR分析Igf-2，Igf-2 r，和H19的等位基因特异性表达的睾丸和卵巢的小鼠之间的种间杂交小家鼠和小家鼠spretus。由于基因组印记，在大多数组织中，Igf-2仅从父本等位基因表达，Igf-2 r和H19仅从母本等位基因表达。虽然保持在新生儿睾丸和卵巢的等位基因特异性表达，放松印迹检测出生后7天在男性和睾丸发育过程中继续。在雌性中，在成年卵巢和卵母细胞中观察到Igf-2和Igf-2 r亲本印记的松弛。这些结果（1）表明，印记表达在配子发生过程中是放松的，可能是印记机制的结果或先决条件，（2）预测随后的印记事件，之后Igf-2，Igf-2 r和H19的等位基因特异性表达反映了起源的亲本。
Successful mammalian development requires both the male and female genomes. This is due in part to genomic imprinting, which results in offspring inheriting only one functional copy of a gene from either the mother or the father. Evidence suggests that this specialization of the parental genomes is established during gametogenesis when the imprint pattern inherited from the parent is switched to reflect the sex of the progeny. We used reverse transcription-PCR to analyze the allele-specific expression of Igf-2, Igf-2r, and H19 in the testes and ovaries of mice derived from an interspecies cross between Mus musculus and Mus spretus. Because of genomic imprinting, Igf-2 is expressed only from the paternal allele and Igf-2r and H19 only from the maternal allele, in most tissues. Although allele-specific expression was maintained in the neonatal testis and ovary, relaxation of imprinting was detected by 7 days after birth in the male and continued during testis development. In the female, relaxation of the Igf-2 and Igf-2r parental imprints was observed in the adult ovary and oocyte. These results (1) indicate that imprinted expression is relaxed during gametogenesis, presumably as a consequence or prerequisite of the imprinting mechanism, and (2) predict a subsequent imprinting event after which the allele-specific expression of Igf-2, Igf-2r, and H19 reflects the parent of origin.