A Distinct Pathway for Polar Exocytosis in Plant Cell Wall Formation
A Distinct Pathway for Polar Exocytosis in Plant Cell Wall Formation
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植物细胞壁形成中极性胞吐作用的独特途径。
DOI：
10.1104/pp.16.00754
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发表时间：
2016
期刊：
影响因子：
7.4
通讯作者：
Jiang Liwen
中科院分区：
文献类型：
--
作者：
Wang Hao;Zhuang Xiaohong;Wang Xiangfeng;Law Angus Ho Yin;Zhao Teng;Du Shengwang;Loy Michael M. T.;Jiang Liwen
一般认为，高尔基体后蛋白分选和运输到质膜（PM）是通过高尔基体反式网络（TGN）发生的。在这项研究中，使用烟草果胶甲酯酶（NtPPME 1）作为标记，我们已经确定了一个TGN独立的极性胞吐途径，介导细胞壁的形成在细胞扩张和胞质分裂。共聚焦免疫荧光和免疫金电子显微镜研究表明，高尔基体衍生的分泌囊泡（GDSV）标记的NtPPME 1-GFP是不同于那些属于传统的后高尔基体胞吐途径的细胞器。此外，药物治疗，超分辨率成像和动态研究表明，NtPPME 1遵循Golgi-GDSV-PM/细胞板（CP）的极性胞吐过程，这与传统的Golgi-TGN-PM/CP分泌途径不同。进一步的研究表明，ROP 1调节这种特定的极性胞吐途径。两者合计，我们已经证明了一种替代的TGN独立的高尔基体到PM极性胞吐途径，介导NtPPME 1的分泌，用于细胞扩增和胞质分裂期间的细胞壁形成，并且是ROP 1依赖的。
Post-Golgi protein sorting and trafficking to the plasma membrane (PM) is generally believed to occur via the trans-Golgi network (TGN). In this study usingNicotiana tabacumpectin methylesterase (NtPPME1) as a marker, we have identified a TGN-independent polar exocytosis pathway that mediates cell wall formation during cell expansion and cytokinesis. Confocal immunofluorescence and immunogold electron microscopy studies demonstrated that Golgi-derived secretory vesicles (GDSVs) labeled by NtPPME1-GFP are distinct from those organelles belonging to the conventional post-Golgi exocytosis pathway. In addition, pharmaceutical treatments, superresolution imaging, and dynamic studies suggest that NtPPME1 follows a polar exocytic process from Golgi-GDSV-PM/cell plate (CP), which is distinct from the conventional Golgi-TGN-PM/CP secretion pathway. Further studies show that ROP1 regulates this specific polar exocytic pathway. Taken together, we have demonstrated an alternative TGN-independent Golgi-to-PM polar exocytic route, which mediates secretion of NtPPME1 for cell wall formation during cell expansion and cytokinesis and is ROP1-dependent.