AN ABC TRANSPORTER IN THE MITOCHONDRIAL INNER MEMBRANE IS REQUIRED FOR NORMAL GROWTH OF YEAST
AN ABC TRANSPORTER IN THE MITOCHONDRIAL INNER MEMBRANE IS REQUIRED FOR NORMAL GROWTH OF YEAST
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DOI：
10.1002/j.1460-2075.1995.tb06989.x
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发表时间：
1995-01-03
期刊：
影响因子：
11.4
通讯作者：
SCHATZ, G
中科院分区：
文献类型：
--
作者：
LEIGHTON, J;SCHATZ, G
为了鉴定线粒体三磷酸腺苷结合盒(ABC)转运蛋白，我们利用聚合酶链式反应从酿酒酵母中扩增出10个与ABC家族成员同源的DNA片段。我们打乱了五个相应的基因，发现其中一个零突变株几乎不能在丰富的培养基上生长，也不能在最低限度的培养基上生长。该基因被称为ATM1，编码694个氨基酸的“半转运体”。Atm1p是通过N端线粒体基质靶向信号合成的，定位于线粒体内膜，其C端ATPase结构域暴露在基质中。缺乏功能性ATM1基因的细胞有一个不稳定的线粒体基因组，并且有完全缺乏细胞色素的白色线粒体。Atm1p是第一个被发现的ABC家族的线粒体成员，也是唯一一个被证明是细胞正常生长所必需的真核ABC转运蛋白。
In an attempt to identify a mitochondrial ATP binding cassette (ABC) transporter, we have used the polymerase chain reaction to amplify 10 DNA fragments homologous to members of the ABC family from the yeast Saccharomyces cerevisiae. We disrupted five of the corresponding genes and found that one of the resulting null mutants barely grew on rich medium and failed to grow on minimal medium. This gene, termed ATM1, encodes a putative 'half-transporter' of 694 amino acids. Atm1p is synthesized with an N-terminal mitochondrial matrix-targeting signal and is located in the mitochondrial inner membrane, with its C-terminal ATPase domain exposed to the matrix. Cells lacking a functional ATM1 gene have an unstable mitochondrial genome and have white mitochondria that completely lack cytochromes. Atm1p is the first mitochondrial member of the ABC family to be identified and the only eukaryotic ABC transporter that has been shown to be necessary for normal cellular growth.