Loss of Runx3 affects ovulation and estrogen-induced endometrial cell proliferation in female mice
Loss of Runx3 affects ovulation and estrogen-induced endometrial cell proliferation in female mice
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DOI：
10.1002/mrd.20904
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发表时间：
2008-11-01
影响因子：
2.5
通讯作者：
Takahashi, Sumio
中科院分区：
文献类型：
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作者：
Sakuma, Atsuko;Fukamachi, Hiroshi;Takahashi, Sumio
Runx3是一种属于Runx家族的转录因子。我们使用Runx3基因敲除（Runx3(-/-)）小鼠研究Runx3在小鼠卵巢和子宫中的功能。取8周龄野生型（wt）和Runx3（-/-）小鼠卵巢。组织学研究表明，在Runx3（-/-）和wt小鼠卵巢中存在不同发育阶段的卵泡。Runx3（-/-）小鼠的原发、前腔和窦腔卵泡数量明显少于wt小鼠，而Runx3（-/-）小鼠的原始卵泡数量与wt小鼠无显著差异。Runx3（-/-）小鼠卵巢未检出黄体。未成熟雌性小鼠的促性腺激素治疗在Runx3（-/-）小鼠和wt小鼠中均能诱导排卵，表明Runx3（-/-）小鼠卵巢对促性腺激素治疗的反应与wt小鼠卵巢相同。提示排卵失败与促性腺激素分泌调节机制功能障碍有关。此外，Runx3（-/-）小鼠的子宫萎缩，上皮层较wt小鼠薄，子宫内膜上皮细胞DNA复制对雌激素无反应。这些结果表明Runx3参与了生殖功能的调节。
Runx3 is a transcription factor that belongs to the Runx family. We studied the function of Runx3 in the mouse ovary and uterus using the Runx3 knockout (Runx3(-/-)) mouse. Ovaries were collected from 8-week-old wild type (wt) and Runx3(-/-) mice. Histological studies showed that follicles were present at various developmental stages in the Runx3(-/-) and wt mouse ovaries. The numbers of primary, preantral and antral follicles in the Runx3(-/-) mice were significantly less than those in the wt mice while the number of primordial follicles in the Runx3(-/-) mice was not significantly different from that in the wt mice. Corpora lutea were not detected in the Runx3(-/-) mouse ovary. Gonadotropin treatment in immature female mice induced ovulation in Runx3(-/-) mice as well as in wt mice, indicating that ovaries of Runx3(-/-) mice respond to gonadotropin treatment as those in wt mouse ovaries. This suggests that failure of ovulation is due to dysfunction of regulatory mechanism of gonadotropin secretion. In addition, the uteri of Runx3(-/-) mice were atrophic, showed thin epithelial layers compared with those of the wt mice, and did not respond to estrogen in terms of DNA replication in endometrial epithelial cells. These results suggest that Runx3 takes part in the regulation of reproductive functions.