Electron donation from cyclic and respiratory flows to the photosynthetic intersystem chain is mediated by pyridine nucleotide dehydrogenase in the cyanobacterium Synechocystis PCC 6803
Electron donation from cyclic and respiratory flows to the photosynthetic intersystem chain is mediated by pyridine nucleotide dehydrogenase in the cyanobacterium Synechocystis PCC 6803
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DOI：
10.1093/oxfordjournals.pcp.a078378
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发表时间：
1992-12
影响因子：
4.9
通讯作者：
H. Mi;T. Endo;U. Schreiber;T. Ogawa;K. Asada
中科院分区：
文献类型：
--
作者：
H. Mi;T. Endo;U. Schreiber;T. Ogawa;K. Asada
将野生型细胞中远红光和强白光脉冲诱导的 P700 氧化还原动力学与蓝藻集胞藻 PCC 6803 的 NAD(P)H 脱氢酶 (NDH) 缺陷突变体中的氧化还原动力学进行比较。野生型细胞显示出来自呼吸供体的电子捐赠和 PS I 中通过 P700+ 产生的光还原剂。 质体醌，由 Hg2+ 敏感酶介导。然而，NDH 缺陷型突变细胞并未表现出通过质体醌从 PS I 中的光还原剂和使用吡啶核苷酸作为中间体的胞质电子供体向 P700+ 的电子转移。因此，NDH 似乎是集胞藻中 NAD(P)H 介导的循环电子流和呼吸电子流中电子主要进入质体醌库的位点。
The redox kinetics of P700 induced by far-red light and a pulse of strong white light in wild type cells were compared with those in NAD(P)H dehydrogenase (NDH)-defective mutants of the cyanobacteriumSynechocystisPCC 6803. The wild type cells showed the electron donation from the respiratory donor and the photoreductant generated in PS I to P700+through the plastoquinone, which is mediated by a Hg2+-sensitive enzyme. The NDH-defective mutant cells, however, did not show the electron transfer to P700+through the plastoquinone from both the photoreductant in PS I and cytosolic electron donors using pyndine nucleotides as an intermediate. Thus, NDH appears to be the site of main entry of electrons into the plastoquinone pool in the NAD(P)H-mediated cyclic electron flow and the respiratory electron flow inSynechocystis.