Effect of 5-hydroxymethylfurfural derived from processed Cornus officinalis on the prevention of high glucose-induced oxidative stress in human umbilical vein endothelial cells and its mechanism
Effect of 5-hydroxymethylfurfural derived from processed Cornus officinalis on the prevention of high glucose-induced oxidative stress in human umbilical vein endothelial cells and its mechanism
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DOI：
10.1016/j.foodchem.2012.11.143
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发表时间：
2013-09-15
期刊：
影响因子：
8.8
通讯作者：
Tu, Sicong
中科院分区：
文献类型：
--
作者：
Cao, Gang;Cai, Hao;Tu, Sicong
本研究旨在探讨5-HMF对体外高糖损伤的人脐静脉内皮细胞（HUVEC）的保护作用及其机制。我们的结果表明，高糖诱导的 HUVEC 氧化应激主要通过活性氧 (ROS)、Jun N 激酶 2/3 (JNK2/3) 和血浆白细胞介素 8 (IL-8) 的激活以及磷酸化蛋白激酶 B (P-Akt) 的失活介导。在 5-HMF（100、200 和 400 μM）存在下，用含有高葡萄糖（4.5%）的培养基处理 HUVEC 会显着抑制高葡萄糖诱导的氧化应激以及 JNK1 和 JNK2/3 的表达。此外，5-HMF 快速抑制高葡萄糖诱导的 P-Akt 下游激活剂 IL-8 的激活。糖尿病可引起多种血管并发症，高糖可诱导血管内皮细胞凋亡。糖尿病中，葡萄糖氧化会大量形成自由基。该研究结果突出了5-HMF在预防心血管和糖尿病疾病方面的药理应用，为进一步开发山茱萸制剂治疗糖尿病相关血管疾病提供了理论基础。 (C) 2013 Elsevier Ltd. 保留所有权利。
The aim of this study was to investigate the protective effect of 5-HMF on human umbilical vein endothelial cells (HUVECs) injured by high glucose in vitro, and the mechanism underlying this process. Our results demonstrated that high glucose-induced oxidative stress in HUVECs was mainly mediated through activation of reactive oxygen species (ROS), Jun N-kinase 2/3 (JNK2/3) and plasma interleukin-8 (IL-8), and inactivation of phosphorylated protein kinase B (P-Akt). Treatment of HUVECs with media containing high glucose (4.5%) in the presence of 5-HMF (100, 200 and 400 mu M) resulted in significant inhibition of high glucose-induced oxidative stress and expression of JNK1 and JNK2/3. Furthermore, 5-HMF rapidly inhibited high glucose-induced activation of IL-8, a downstream activator of P-Akt. Diabetes mellitus can cause a wide variety of vascular complications and high glucose can induce vascular endothelial cell apoptosis. Free radicals are formed disproportionately in diabetes by glucose oxidation. The finding of this study highlights the pharmacological application of 5-HMF for preventing cardiovascular and diabetes mellitus diseases, and provides the theoretical basis for further development of a Cornus officinalis agent for diabetes-associated vascular diseases. (C) 2013 Elsevier Ltd. All rights reserved.