Evaluation of genotypic diversity of Streptococcus mutans using distinct arbitrary primers
Evaluation of genotypic diversity of Streptococcus mutans using distinct arbitrary primers
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DOI：
10.1590/s1678-77572008000600009
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发表时间：
2008-12-01
影响因子：
2.7
通讯作者：
Cury, Jaime Aparecido
中科院分区：
文献类型：
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作者：
Tabchoury, Cínthia Pereira Machado;Sousa, Maria Clara K.;Cury, Jaime Aparecido
变形链球菌一直被认为是龋病的主要病原菌之一，其毒力因素可能是其基因多样性，而不是唾液计数。在用随机引物进行聚合酶链式反应(PCR)基因分型时，为了提高扩增产物图谱的复杂性和特异性，已经使用了几个引物。因此，本研究的目的是评价从唾液中分离的5个不同的随机引物进行AP-PCR反应时，基因分型的符合度。用OPA02、03、05、13、18的随机引物对11名成年志愿者的唾液标本进行分离，对88株变形链球菌进行基因分型。大多数志愿者(11人中有9人)只有一种基因型。本研究结果表明，OPA02、03、05和13四对引物适合于成人志愿者唾液中变形链球菌的基因分型。
Streptococcus mutans has been considered one of the main etiological agents of dental caries and the genotypic diversity rather than its salivary counts may be considered as a virulence factor of this bacterium. For genotyping with polymerase chain reaction (PCR) with arbitrary primers, several primers have been used in order to improve complexity and specificity of amplicon patterns. Thus, the aim of this study was to evaluate the degree of agreement of genotypic identification among AP-PCR reactions performed with 5 distinct arbitrary primers of S. mutans isolated from saliva. Stimulated saliva was collected from 11 adult volunteers for isolation of S. mutans, and a total of 88 isolates were genotyped with arbitrary primers OPA 02, 03, 05, 13 and 18. Fourteen distinct genotypes were identified in the saliva samples. Most volunteers ( 9 out of 11) presented only one genotype. The results of the present study suggest that primers OPA 02, 03, 05 and 13 were suitable for genotypic identification of S. mutans isolates of saliva from adult volunteers.