Identification of genes preferentially expressed in periodontal ligament: Specific expression of a novel secreted protein, FDC-SP
Identification of genes preferentially expressed in periodontal ligament: Specific expression of a novel secreted protein, FDC-SP
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DOI：
10.1016/j.bbrc.2005.10.076
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发表时间：
2005-12-16
影响因子：
3.1
通讯作者：
Shinomura, T
中科院分区：
文献类型：
--
作者：
Nakamura, S;Terashima, T;Shinomura, T
通过抑制性消减杂交检测人牙周膜（PDL）中的基因表达，以鉴定与培养的PDL成纤维细胞相比在组织中优先表达的基因。消减cDNA文库中最富集的基因主要是细胞外基质组分、I型和III型胶原蛋白、光蛋白聚糖、骨膜蛋白和asporin等的基因，其表达将独特的机械性质传递给PDL。在这一组中还有滤泡树突细胞分泌蛋白（FDC-SP）的基因，这是一种类似唾液中的statherin的小蛋白，以前在PDL中没有发现。FDC-SP在PDL中的存在通过小鼠中的原位杂交证实，其还显示FDC-SP明确存在于腮腺中，但令人惊讶的是，不在其他唾液腺中：下颌下和舌下。由于只检查了正常组织，这些发现表明FDC-SP在口腔结缔组织中起着重要但以前未被怀疑的作用。(c)2005年爱思唯尔公司All rights reserved.
Gene expression in human periodontal ligament (PDL) was examined by suppression subtractive hybridization to identify genes that are preferentially expressed in tissue compared to cultured PDL fibroblasts. The most enriched genes in a subtracted cDNA library are primarily genes for extracellular matrix components, types I and III collagen, lumican, periostin, and asporin, among others, whose expression conveys unique mechanical properties to the PDL. Also within this group is the gene for follicular dendritic cell secreted protein (FDC-SP), a small protein like statherin in saliva, not previously found in PDL. FDC-SP's presence in PDL was confirmed by in situ hybridization in mouse which also showed that it was definitely present in the parotid gland, but, surprisingly, not in the other salivary glands: submandibular and sublingual. Since only normal tissue was examined, these findings suggest that FDC-SP plays an important but previously unsuspected role within oral connective tissue. (c) 2005 Elsevier Inc. All rights reserved.