Copper-free dual labeling of DNA by triazines and cyclopropenes as minimal orthogonal and bioorthogonal functions† †Electronic supplementary information (ESI) available: Synthesis of 1, images of NMR and mass spectra, images of PAGE analyses after primer extension. See DOI: 10.1039/c8sc05588b
Copper-free dual labeling of DNA by triazines and cyclopropenes as minimal orthogonal and bioorthogonal functions† †Electronic supplementary information (ESI) available: Synthesis of 1, images of NMR and mass spectra, images of PAGE analyses after primer extension. See DOI: 10.1039/c8sc05588b
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DOI：
10.1039/c8sc05588b
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发表时间：
2019-03
期刊：
影响因子：
8.4
通讯作者：
Ulrike Reisacher;Damian Ploschik;Franziska Rönicke;G. Cserép;P. Kele;H. Wagenknecht
中科院分区：
文献类型：
--
作者：
Ulrike Reisacher;Damian Ploschik;Franziska Rönicke;G. Cserép;P. Kele;H. Wagenknecht
应用两个不同的小功能的反电子需求diols - alder反应对DNA进行双重标记：1,2,4-三嗪连接到2 ' -脱氧尿嘧啶的5位，1-甲基环丙烯连接到7-二氮杂-2 ' -脱氧腺苷的7位。
Two different and small functions for inverse electron demand Diels–Alder reactions were applied for dual labeling of DNA: the 1,2,4-triazine was attached to the 5-position of 2′-deoxyuridine, and the 1-methylcyclopropene to the 7-position of 7-deaza-2′-deoxyadenosine.