Vagal Sensory Neuron Subtypes that Differentially Control Breathing.
Vagal Sensory Neuron Subtypes that Differentially Control Breathing.
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DOI：
10.1016/j.cell.2015.03.022
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发表时间：
2015-04-23
期刊：
影响因子：
64.5
通讯作者：
Liberles SD
中科院分区：
文献类型：
--
作者：
Chang RB;Strochlic DE;Williams EK;Umans BD;Liberles SD
呼吸是生存所必需的，并受到精确的神经控制。迷走神经是正常呼吸所需的肺和脑之间的主要管道。在这里，我们确定了两个群体的小鼠迷走神经传入（P2ry1，Npy2r），每几百个神经元，对呼吸产生强大的和相反的影响。遗传引导的解剖映射显示，这些神经元密集地支配肺，并向不同的脑干靶点发送长距离投射。Npy2r神经元主要是慢传导C纤维，而P2ry1神经元主要是接触肺内分泌细胞（神经上皮体）的快传导A纤维。P2ry1神经元的光遗传刺激会使呼吸急剧沉默，使动物陷入呼气中，而刺激Npy2r神经元会导致快速、浅呼吸。激活P2ry1神经元不影响心率或胃压，其他自主神经功能下迷走神经控制。因此，迷走神经包含混合的感觉神经元，其构成具有不同解剖学连接和生理学作用的遗传上可定义的标记线。
Breathing is essential for survival and under precise neural control. The vagus nerve is a major conduit between lung and brain required for normal respiration. Here, we identify two populations of mouse vagus nerve afferents (P2ry1, Npy2r), each a few hundred neurons, that exert powerful and opposing effects on breathing. Genetically guided anatomical mapping revealed that these neurons densely innervate the lung and send long-range projections to different brainstem targets. Npy2r neurons are largely slow-conducting C fibers, while P2ry1 neurons are largely fast-conducting A fibers that contact pulmonary endocrine cells (neuroepithelial bodies). Optogenetic stimulation of P2ry1 neurons acutely silences respiration, trapping animals in exhalation, while stimulating Npy2r neurons causes rapid, shallow breathing. Activating P2ry1 neurons did not impact heart rate or gastric pressure, other autonomic functions under vagal control. Thus, the vagus nerve contains intermingled sensory neurons constituting genetically definable labeled lines with different anatomical connections and physiological roles.