Analysis of circulating regulatory T cells (CD4+CD25+CD127-) after cryosurgery in prostate cancer
Analysis of circulating regulatory T cells (CD4+CD25+CD127-) after cryosurgery in prostate cancer
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DOI：
10.1038/aja.2013.22
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发表时间：
2013-07-01
影响因子：
2.9
通讯作者：
Guo, Zhi
中科院分区：
文献类型：
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作者：
Si, Tong-Guo;Wang, Jun-Ping;Guo, Zhi
本研究通过测量调节性T细胞（Tregs）的频率和免疫功能来评估前列腺癌（PCa）患者冷冻手术后调节性T细胞（Tregs）的反应。在30例接受冷冻手术的高危PCa患者和15名健康志愿者的治疗前、治疗后4周和8周采集血液。分离循环CD4(+)CD25(+)CD127(-) Tregs。通过流式细胞术检测其频率，并通过测量与Tregs共培养的CD4(+)CD25(-) T细胞的增殖来评估其免疫抑制功能。结果显示，与健康志愿者相比，PCa患者的循环CD4(+)CD25(+)CD127(-) Tregs百分比增加(7.6%+/- 0.73% vs. 5.8%+/- 0.54%， P
This study was performed to assess the response of regulatory T cells (Tregs) following cryosurgery in prostate cancer (PCa) patients by measuring their frequency and immune function. Blood was collected prior to and at 4 and 8 weeks after treatment in 30 patients with high-risk PCa who underwent cryosurgery and from 15 healthy volunteers. Circulating CD4(+)CD25(+)CD127(-) Tregs were isolated. Their frequency was detected by flow cytometry, and immune suppressive function was evaluated by measuring the proliferation of CD4(+)CD25(-) T cells cocultured with Tregs. The results showed that the percentage of circulating CD4(+)CD25(+)CD127(-) Tregs was increased in PCa patients compared to healthy volunteers (7.6%+/- 0.73% vs. 5.8%+/- 0.54%, P