FOCAL POINTS FOR CHROMOSOME CONDENSATION AND DECONDENSATION REVEALED BY 3-DIMENSIONAL INVIVO TIME-LAPSE MICROSCOPY
FOCAL POINTS FOR CHROMOSOME CONDENSATION AND DECONDENSATION REVEALED BY 3-DIMENSIONAL INVIVO TIME-LAPSE MICROSCOPY
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DOI：
10.1038/342293a0
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发表时间：
1989-11-16
期刊：
影响因子：
64.8
通讯作者：
AGARD, DA
中科院分区：
文献类型：
--
作者：
HIRAOKA, Y;MINDEN, JS;AGARD, DA
尽管在有丝分裂过程中，染色体在凝聚状态下的动态行为已经被广泛研究，但染色体在进入和离开间期的行为还没有很好的文献记载。以往的电子显微镜研究表明，染色体在核外周以不均匀的方式凝聚1，2。但染色体凝聚是一个复杂的动态过程，需要在活组织中持续观察才能完全了解。使用最近发展的三维延时荧光显微镜技术，我们观察到染色体从末期开始松弛，经过间期，直到下一个前期凝聚。通过实施新的赤平投影和计算处理协议，这项技术已得到改进，以产生更高分辨率的图像4。这些研究表明，核膜上不同于着丝粒和端粒的染色体区域是二倍体染色体解聚和凝聚的焦点。相间初期后期冷凝中心的相对位置似乎与后续前期的早期冷凝中心相对应。
ALTHOUGH the dynamic behaviour of chromosomes has been extensively studied in their condensed state during mitosis, chromosome behaviour during the transition to and from interphase has not been well documented. Previous electron microscopic studies suggest that chromosomes condense in a non-uniform fashion at the nuclear periphery1,2. But chromosome condensation is a complicated and dynamic process and requires continuous observation in living tissues to be fully understood. Using a recently developed three-dimensional time-lapse fluorescence microscopy technique3, we have observed chromosomes as they relax from telophase, through interphase, until their condensation at the next prophase. This technique has been improved to produce higher-resolution images by implementing new stereographic projection and computational processing protocols4. These studies have revealed that chromosomal regions on the nuclear envelope, distinct from the centromeres and telomeres, serve as foci for the decondensation and condensation of diploid chromosomes. The relative positions of the late decondensation sites at the beginning of interphase appear to correspond to the early condensation sites at the subsequent prophase.