Human cytomegalovirus miR-US33-5p inhibits viral DNA synthesis and viral replication by down-regulating expression of the host Syntaxin3
Human cytomegalovirus miR-US33-5p inhibits viral DNA synthesis and viral replication by down-regulating expression of the host Syntaxin3
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DOI：
10.1016/j.febslet.2014.12.030
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发表时间：
2015-02-13
期刊：
影响因子：
3.5
通讯作者：
Ruan, Qiang
中科院分区：
文献类型：
--
作者：
Guo, Xin;Qi, Ying;Ruan, Qiang
在人巨细胞病毒(HCMV)感染过程中，高表达的HCMV-miR-US33可抑制病毒的裂解复制，下调US29基因的表达。然而，目前尚不清楚miR-US33对病毒复制的抑制是通过下调US29的表达还是通过下调其他宿主基因的表达来实现的。在这里，我们利用杂交-聚合酶链式反应和荧光素酶报告基因分析，确定宿主基因Synaxin3(STX3)是HCMV-miR-US33-5P的直接靶标。进一步证明，在HCMV-miR-US33-5p过表达细胞中，STX3蛋白水平下调。用STX3特异的siRNA或HCMV-miR-US33-5p的抑制剂进行的实验证实，HCMV-miR-US33-5p介导的抑制HCMV DNA合成和病毒复制的作用是通过下调STX3的表达而特异性地介导的。(C)2015年欧洲生化学会联合会。爱思唯尔出版，版权所有。
During infection with human cytomegalovirus (HCMV), overexpression of hcmv-miR-US33 can inhibit the lytic viral replication and down-regulate US29 mRNA. However, it remains unknown whether inhibition of viral replication by miR-US33 is mediated by down-regulation of expression of US29 or another host gene. Here, we identified the host gene Syntaxin3 (STX3) to be a direct target of hcmv-miR-US33-5p using Hybrid-PCR and luciferase-reporter assays. It was further demonstrated that the levels of STX3 protein were down-regulated in hcmv-miR-US33-5p-overexpressing cells. Experiments with STX3-specific siRNA, or with an inhibitor of hcmv-miR-US33-5p confirmed that hcmv-miR-US33-5p-mediated inhibition of HCMV DNA synthesis and of viral replication are specifically mediated by down-regulation of STX3 expression. (C) 2015 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.