Unraveling oligodendrocyte origin and function by cell-specific transgenesis
Unraveling oligodendrocyte origin and function by cell-specific transgenesis
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DOI：
10.1159/000048712
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发表时间：
2001-07-01
影响因子：
2.9
通讯作者：
Gallo, V
中科院分区：
文献类型：
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作者：
Belachew, S;Yuan, XQ;Gallo, V
除了成熟的少突胶质细胞在中枢神经系统（CNS）发育过程中髓磷脂合成中的作用外，少突胶质细胞谱系还包含最大的出生后增殖祖细胞库，其体内行为在健康和疾病中仍然广泛难以捉摸。我们在这里描述的转基因模型使我们能够通过使用蛋白脂质蛋白和2',3'-环核苷酸T-磷酸二酯酶（CNP）基因启动子来指导不同记者的少突胶质细胞表达，特别是绿色荧光蛋白（GFP）来跟踪此类细胞的功能和起源。我们强调，CNP-GFP 小鼠以从胚胎到成年的整个少突胶质细胞谱系为目标，为研究正常和受损中枢神经系统中少突胶质细胞祖细胞的体内特性提供了一个出色的工具。版权所有 (C) 2001 S. Karger AG，巴塞尔。
Besides the role of mature oligodendrocytes in myelin synthesis during the development of the central nervous system (CNS), the oligodendrocyte lineage also encompasses the largest pool of postnatal proliferating progenitors whose behavior in vivo remains broadly elusive in health and disease. We describe here transgenic models that allow us to track the functions and origins of such cells by using proteolipid protein and 2',3'-cyclic nucleotide T-phosphodiesterase (CNP) gene promoters to direct oligodendroglial expression of different reporters, in particular the green fluorescent protein (GFP). We emphasize that the CNP-GFP mouse, which targets the entire oligodendroglial lineage from embryonic life to adulthood, provides an outstanding tool to study the in vivo properties of oligodendrocyte progenitor cells in normal and damaged CNS. Copyright (C) 2001 S. Karger AG, Basel.