Isolation and regional localization of DNA segments revealing polymorphic loci from human chromosome 13.
Isolation and regional localization of DNA segments revealing polymorphic loci from human chromosome 13.
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DOI：
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发表时间：
1984
影响因子：
9.8
通讯作者：
W. Cavenee;R. Leach;T. Mohandas;P. Pearson;R. White
中科院分区：
文献类型：
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作者：
W. Cavenee;R. Leach;T. Mohandas;P. Pearson;R. White
从含有人13、12和6p染色体的仓鼠-人体细胞杂种中构建了富集人13号染色体部分的重组DNA文库。共鉴定出733个含有人类DNA插入片段的DNA片段，并衍生出46个单拷贝亚片段，并将其用作从无关个体分离的基因组DNA的Southern转移的探针。从这一组，9个片段揭示多态性位点（RFLP）在Msp I或Taq I消化的DNA已被确定，其中三个是多态性与两种酶。这些探针中的六个已被证明在体细胞杂交作图面板中与人13号染色体一致地分离，并且这些位点处的RFLP已被证明表现为共显性孟德尔等位基因。此外，与从含有13号染色体的各种缺失的细胞分离的DNA的杂交允许区域定位。该重组DNA文库将有助于视网膜母细胞瘤的研究以及该常染色体异常机制的研究。
A recombinant DNA library enriched for portions of human chromosome 13 has been constructed from a hamster-human somatic cell hybrid that contained human chromosomes 13, 12, and 6p. A total of 733 phages were identified that contain human DNA inserts, and 46 single-copy subfragments have been derived and used as probes on Southern transfers of genomic DNA isolated from unrelated individuals. From this set, nine fragments revealing polymorphic loci (RFLP) in Msp I- or Taq I-digested DNA have been identified, of which three are polymorphic with both enzymes. Six of these probes have been shown to segregate concordantly with human chromosome 13 in a somatic cell hybrid mapping panel, and the RFLPs at these loci have been shown to behave as codominant Mendelian alleles. Additionally, hybridization to DNA isolated from cells containing various deletions of chromosome 13 has allowed regional localization. This recombinant DNA library will be useful in the study of retinoblastoma as well as in the study of the mechanisms responsible for abnormalities of this autosome.