THE MORPHOLOGY AND DISTRIBUTION OF KOLMER-AGDUHR CELLS, A CLASS OF CEREBROSPINAL-FLUID-CONTACTING NEURONS REVEALED IN THE FROG EMBRYO SPINAL-CORD BY GABA IMMUNOCYTOCHEMISTRY
THE MORPHOLOGY AND DISTRIBUTION OF KOLMER-AGDUHR CELLS, A CLASS OF CEREBROSPINAL-FLUID-CONTACTING NEURONS REVEALED IN THE FROG EMBRYO SPINAL-CORD BY GABA IMMUNOCYTOCHEMISTRY
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DOI：
10.1098/rspb.1987.0068
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发表时间：
1987-11-23
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影响因子：
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通讯作者：
STORMMATHISEN, J
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作者：
DALE, N;ROBERTS, A;STORMMATHISEN, J
用免疫细胞化学方法定位GABA在戊二醛固定的组织中的表达，已被应用于异种胚胎脊髓的研究。这个程序染色的解剖类神经元，其中有胞体形成两个或多或少连续的行，中央管的两侧，在脊髓的腹侧部分。37-38期胚胎脊髓中染色的神经元总数约为300个。索马体的内侧面突出于中央管，有刷状缘，电镜下可见由许多微绒毛或静纤毛和1 ~ 2根纤毛组成。神经元索马体外端有同侧上行轴突。许多神经元的轴突有一个生长锥，也被清楚地染色。我们建议以W. Kolmer和E. Agduhr描述了它们在许多脊椎动物的脊髓中。他们的早期胚胎起源，GABA样免疫反应，轴突的预测和分布作为一个整体的人口还没有以前已知的。
An immunocytochemical method that localizes GABA in glutaraldehyde- fixed tissue has been applied to the study of theXenopusembryo spinal cord. This procedure stained an anatomical class of neuron, which had somata forming two more or less continuous rows, one on either side of the central canal, in the ventral part of the spinal cord. The total number of stained neurons in the stage 37–38 embryo spinal cord was about 300. The medial surface of the soma protruded into the central canal and had a brush border which electron microscope studies showed to consist of many microvilli or stereocilia and one or two cilia. The external end of the neuron soma had an ipsilateral ascending axon. The axon of many of these neurons had a growth cone which was also clearly stained. We propose calling these neurons ‘Kolmer–Agduhr cells’ after W. Kolmer and E. Agduhr who described them in the spinal cords of many vertebrate classes. Their early embryonic origin, GABA-like immunoreactivity, axonal projections and distribution as a whole population have not previously been known.