Fluorescence probe partitioning between Lo/Ld phases in lipid membranes
Fluorescence probe partitioning between Lo/Ld phases in lipid membranes
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DOI：
10.1016/j.bbamem.2007.05.012
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发表时间：
2007-09-01
影响因子：
3.4
通讯作者：
Feigenson, Gerald W.
中科院分区：
文献类型：
--
作者：
Baumgart, Tobias;Hunt, Geoff;Feigenson, Gerald W.
荧光显微镜成像是研究脂质膜的重要技术，并且越来越多地用于检查脂质双层膜，特别是那些显示宏观共存域的膜。脂相共存是一种具有潜在生物学意义的现象。通过荧光显微镜识别脂质膜异质性依赖于具有明确划分行为的膜标记。虽然荧光团在凝胶相和液体无序相之间的分配已被广泛表征，但对于共存的液相却并非如此。我们使用荧光显微镜成像来检查多种脂质膜标记物在具有各种脂质成分的膜中的液相分配。发现大多数荧光脂质类似物强烈分配到液体无序 (L-d) 相中。相比之下，一些具有平环系统的荧光多环芳烃被发现均匀分配，但其他的优先分配成液态有序（L-o）相。我们发现这些荧光标记物可有效识别由磷脂和胆固醇组成的三元脂质系统中共存的宏观膜相。 (C) 2007 Elsevier B.V. 保留所有权利。
Fluorescence microscopy imaging is an important technique for studying lipid membranes and is increasingly being used for examining lipid bilayer membranes, especially those showing macroscopic coexisting domains. Lipid phase coexistence is a phenomenon of potential biological significance. The identification of lipid membrane heterogeneity by fluorescence microscopy relies on membrane markers with well-defined partitioning behavior. While the partitioning of fluorophores between gel and liquid-disordered phases has been extensively characterized, the same is not true for coexisting liquid phases. We have used fluorescence microscopy imaging to examine a large variety of lipid membrane markers for their liquid phase partitioning in membranes with various lipid compositions. Most fluorescent lipid analogs are found to partition strongly into the liquid-disordered (L-d) phase. In contrast, some fluorescent polycyclic aromatic hydrocarbons with a flat ring system were found to partition equally, but others partition preferentially into liquid-ordered (L-o) phases. We have found these fluorescent markers effective for identification of coexisting macroscopic membrane phases in ternary lipid systems composed of phospholipids and cholesterol. (C) 2007 Elsevier B.V. All rights reserved.