Paracrine regulation of theca androgen production by granulosa cells in the ovary.
Paracrine regulation of theca androgen production by granulosa cells in the ovary.
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DOI：
10.1016/j.fertnstert.2013.04.016
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发表时间：
2013-08
影响因子：
6.7
通讯作者：
Shimasaki, Shunichi
中科院分区：
文献类型：
--
作者：
Hoang, Yvonne D.;McTavish, Kirsten J.;Chang, R. Jeffrey;Shimasaki, Shunichi
目的：检测卵泡刺激素(FSH)刺激颗粒细胞(GC)后，抑制素是否在多大程度上介导卵泡膜细胞(TC)细胞色素P17基因的表达。体外和体外实验研究。加州大学圣地亚哥分校医学院生殖医学系。幼龄雌性SD大鼠。用卵泡刺激素、抑制素、抑制素抗体或β-葡聚糖抗体处理卵巢组织和含或不含GC的体外培养的卵泡膜间质细胞。作为雄激素产生的关键酶，用实时荧光定量RT-PCR检测了细胞色素P17基因的表达水平。24小时后，卵泡刺激素呈剂量依赖性地上调卵巢组织和抽搐组织中细胞色素P17基因的表达，提示卵泡刺激素诱导的卵巢癌细胞分泌的旁分泌因子(S)介导了卵泡刺激素诱导的细胞色素P17基因表达。抗抑制素及抑制素共受体抗体β-Glycan可阻断FSH对细胞色素P17基因表达的刺激作用。然而，单独使用抑制素并不能同样程度地增加细胞色素P17基因的表达水平。这些发现提示抑制素在FSH影响的GC对TC-CYP17基因表达的旁分泌调节中起作用；然而，GCs通过FSH产生的其他旁分泌因子似乎是必需的。
To test whether and to what extent inhibin mediates Cyp17 mRNA expression in theca cells (TCs) in response to FSH stimulation of granulosa cells (GCs). Ex-vivo and in vitro experimental study. Department of Reproductive Medicine, University of California, San Diego, School of Medicine. Immature female Sprague Dawley rats. Ovarian tissue explants and isolated theca-interstitial cell (TIC) preparations with or without GCs were treated with FSH, inhibin, inhibin antibody or β-glycan antibody. As a key enzyme in androgen production, Cyp17 mRNA levels were measured by real-time RT-PCR. After 24 hours, Cyp17 mRNA expression was dose-dependently increased by FSH in ovarian tissue explants and TICs, suggesting that paracrine factor(s) secreted from GCs in response to FSH mediates Cyp17 mRNA expression in TCs. Antibodies against inhibin and inhibin co-receptor, β-glycan, blocked the stimulatory effect of FSH on Cyp17 mRNA expression. However, inhibin alone did not increase Cyp17 mRNA level to the same extent. These findings suggest a role for inhibin in the paracrine regulation of TC Cyp17 mRNA expression by GCs influenced by FSH; however, other paracrine factors produced by GCs by virtue of FSH seem to be required.
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影响因子：
3.6
作者：
Dharia, S;Slane, A;Parker, CR
通讯作者：
Parker, CR
影响因子：
3.6
作者：
Huang, CTF;Weitsman, SR;Magoffin, DA
通讯作者：
Magoffin, DA
DOI：
10.1073/pnas.122066899
发表时间：
2002-06-11
影响因子：
11.1
作者：
Otsuka, F;Shimasaki, S
通讯作者：
Shimasaki, S
影响因子：
4.8
作者：
CARA, JF;ROSENFIELD, RL
通讯作者：
ROSENFIELD, RL
DOI：
10.1073/pnas.82.21.7217
发表时间：
1985-01-01
影响因子：
11.1
作者：
LING, N;YING, SY;GUILLEMIN, R
通讯作者：
GUILLEMIN, R