RADIOSENSITIVITY OF CD4 OR CD8 POSITIVE HUMAN LYMPHOCYTES-T BY AN INVITRO COLONY FORMATION ASSAY
RADIOSENSITIVITY OF CD4 OR CD8 POSITIVE HUMAN LYMPHOCYTES-T BY AN INVITRO COLONY FORMATION ASSAY
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DOI：
10.2307/3577549
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发表时间：
1990-08-01
影响因子：
3.4
通讯作者：
AKIYAMA, M
中科院分区：
文献类型：
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作者：
NAKAMURA, N;KUSUNOKI, Y;AKIYAMA, M
最近发展的体外淋巴细胞集落测定法使得有可能使用外周血淋巴细胞（PBL）而不是皮肤成纤维细胞测定法来检查人的辐射敏感性的变化。我们最近的研究（M。Hakoda等人，穆塔Res. 197，161-169，1988）显示，大多数集落由携带CD 4或CD 8抗原的淋巴细胞组成。由于PBL中CD 4+和CD 8+细胞的比例在个体之间不同，我们怀疑如果CD 4+和CD 8+细胞的剂量-存活曲线彼此不同，则个体的放射敏感性可能由于不同的亚群频率而偏倚。在本研究中，CD 4+（辅助/诱导T细胞）和CD 8+（抑制/细胞毒性T细胞）淋巴细胞分离的PBL和它们的剂量生存曲线进行了测定。结果表明，这两种类型细胞的D10（将存活分数降低至10%所需的剂量）相似[3.13 . ±. 0.10戈伊（平均值±. SD），CD4+，3.34 ±。0.50戈伊（CD 8+）和3.14 .+-。0.17戈伊用于未分选的细胞]，支持使用整个PBL群体来筛选具有改变的放射敏感性的个体。
The recent development of an in vitro lymphocyte colony assay makes it possible to examine variations in the radiosensitivity of humans using peripheral blood lymphocytes (PBL) instead of the skin fibroblast assay. Our recent study (M. Hakoda et al., Mutat. Res. 197, 161-169, 1988) showed that most of the colonies consisted of lymphocytes bearing CD4 or CD8 antigens. Since the fraction of CD4+ and CD8+ cells in PBL differs among individuals, we suspected that individual radiosensitivity might be biased by the different subset frequencies if the dose-survival curves of the CD4+ and CD8+ cells were different from each other. In the present study, CD4+ (helper/inducer T) and CD8+ (suppressor/cytotoxic T) lymphocytes were isolated from PBL and their dose-survival curves were determined. The results showed that the D10 (dose required to reduce the surviving fraction to 10%) was similar for these two types of cells [3.13 .+-. 0.10 Gy (mean .+-. SD) for CD4+, 3.34 .+-. 0.50 Gy for CD8+ and 3.14 .+-. 0.17 Gy for the unsorted cells], supporting the use of the whole PBL population for the screening of individuals with altered radiosensitivity.