MYELIN MEMBRANE BIOGENESIS BY OLIGODENDROCYTES - DEVELOPMENTAL REGULATION OF LOW-MOLECULAR-WEIGHT GTP-BINDING PROTEINS
MYELIN MEMBRANE BIOGENESIS BY OLIGODENDROCYTES - DEVELOPMENTAL REGULATION OF LOW-MOLECULAR-WEIGHT GTP-BINDING PROTEINS
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DOI：
10.1016/0014-5793(94)00562-1
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发表时间：
1994-06-27
期刊：
影响因子：
3.5
通讯作者：
PFEIFFER, SE
中科院分区：
文献类型：
--
作者：
HUBER, LA;MADISON, DL;PFEIFFER, SE
少突胶质细胞合成大量的髓鞘膜。我们假设这需要囊泡运输的独特方面。测定了少突胶质细胞谱系的特定阶段中涉及囊泡转运途径调节的低分子量GTP结合蛋白（二维凝胶电泳，[α-P-32]GTP覆盖）。与该假设一致，当少突胶质细胞从早期祖细胞分化为成熟的髓鞘生成细胞时，大于或等于12个小GTP结合蛋白被上调。髓磷脂膜也具有复杂的GTP结合蛋白模式。这些蛋白质中的一些可能是特定的少突胶质细胞，这表明少突胶质细胞可能利用细胞类型特异性GTP结合蛋白的髓鞘膜的生物发生和维护。
Oligodendrocytes synthesize dramatic amounts of myelin membrane. We hypothesized that this requires unique aspects of vesicular trafficking. Specific stages of the oligodendrocyte lineage were assayed for low molecular weight GTP-binding proteins implicated in the regulation of vesicular transport pathway (two dimensional gel electrophoresis, [alpha-P-32]GTP overlay). Consistent with the hypothesis, as oligodendrocytes differentiate from early progenitors to mature myelin-producing cells, greater than or equal to 12 small GTP-binding proteins become up-regulated. Myelin membrane also has a complex pattern of GTP-binding proteins. Several of these proteins may be specific to oligodendrocytes, suggesting that oligodendrocytes may utilize cell-type specific GTP-binding proteins for biogenesis and maintenance of the myelin membrane.