Genome and transcriptome of a pathogenic yeast, Candida nivariensis.
Genome and transcriptome of a pathogenic yeast, Candida nivariensis.
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DOI：
10.1093/g3journal/jkab137
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发表时间：
2021-07-14
期刊：
影响因子：
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通讯作者：
Timp W
中科院分区：
文献类型：
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作者：
Fan Y;Gale AN;Bailey A;Barnes K;Colotti K;Mass M;Morina LB;Robertson B;Schwab R;Tselepidakis N;Timp W
我们提出了一个高度连续的基因组和转录组的致病性酵母菌，念珠菌。我们使用牛津纳米孔技术和Illumina平台对该物种的DNA和RNA进行了测序。我们将基因组组装成11.8 Mb的草图，由16个contigs组成，N50为886 Kb，其中包括一个28 Kb的圆形线粒体序列。利用直接RNA纳米孔测序和Illumina cDNA测序，我们构建了新组装体的注释，并补充了来自酿酒酵母（Saccharomyces cerevisiae）和心念珠菌（Candida glabrata）的基因。
We present a highly contiguous genome and transcriptome of the pathogenic yeast, Candida nivariensis. We sequenced both the DNA and RNA of this species using both the Oxford Nanopore Technologies and Illumina platforms. We assembled the genome into an 11.8 Mb draft composed of 16 contigs with an N50 of 886 Kb, including a circular mitochondrial sequence of 28 Kb. Using direct RNA nanopore sequencing and Illumina cDNA sequencing, we constructed an annotation of our new assembly, supplemented by lifting over genes from Saccharomyces cerevisiae and Candida glabrata.