Metabolic pathways in Anopheles stephensi mitochondria.
Metabolic pathways in Anopheles stephensi mitochondria.
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Anopheles Stephensi线粒体中的代谢途径。
DOI：
10.1042/bj20080973
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发表时间：
2008-10-15
期刊：
影响因子：
--
通讯作者：
Luckhart S
中科院分区：
文献类型：
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作者：
Giulivi C;Ross-Inta C;Horton AA;Luckhart S
尚未对疟疾病媒蚊子的线粒体进行研究。这一信息将有助于了解蚊子的衰老和杀虫剂的解毒作用，这两个参数对疟疾流行地区的疟原虫传播有重大影响。在这里，我们报告的分析呼吸和氧化磷酸化的线粒体培养细胞（ASE线）从斯氏按蚊，疟疾的主要载体在印度，东南亚和中东部分地区。ASE细胞线粒体与哺乳动物肌肉线粒体有许多共同的特征，尽管这些细胞具有幼虫起源。然而，与哺乳动物线粒体的两个主要差异是明显的。第一，甘油-磷酸穿梭在ASE细胞线粒体中的NADH氧化中起主要作用，就像在昆虫肌肉线粒体中一样。相比之下，哺乳动物白色肌肉线粒体主要依赖于乳酸脱氢酶，而红色肌肉线粒体依赖于苹果酸-草酰乙酸穿梭。第二，ASE线粒体能够以与α-甘油磷酸相当的速率氧化Pro。然而，Pro途径似乎与目前公认的途径不同，因为酮戊二酸可以通过克雷布斯循环或通过转氨作用完全分解，这取决于ATP的需要。
No studies have been performed on mitochondria of malaria vector mosquitoes. This information would be valuable in understanding mosquito aging and detoxification of insecticides, two parameters that significantly impact malaria parasite transmission in endemic regions. Here, we report the analyses of respiration and oxidative phosphorylation in mitochondria of cultured cells (ASE line) from Anopheles stephensi, a major vector of malaria in India, Southeast Asia and parts of the Middle East. ASE cell mitochondria shared many features in common with mammalian muscle mitochondria, despite the fact that these cells have a larval origin. However, two major differences with mammalian mitochondria were apparent. One, the glycerol-phosphate shuttle plays a major role in NADH oxidation in ASE cell mitochondria as it does in insect muscle mitochondria. In contrast, mammalian white muscle mitochondria depend primarily on lactate dehydrogenase, whereas red muscle mitochondria depend on the malate-oxaloacetate shuttle. Two, ASE mitochondria were able to oxidize Pro at a rate comparable with that of α-glycerophosphate. However, the Pro pathway appeared to differ from the currently accepted pathway, in that ketoglutarate could be catabolyzed completely by the Krebs cycle or via transamination depending on the ATP need.