IDENTIFICATION OF 2 HUMAN BETA-TUBULIN ISOTYPES
IDENTIFICATION OF 2 HUMAN BETA-TUBULIN ISOTYPES
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DOI：
10.1128/mcb.3.5.854
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发表时间：
1983-01-01
影响因子：
5.3
通讯作者：
COWAN, NJ
中科院分区：
文献类型：
--
作者：
HALL, JL;DUDLEY, L;COWAN, NJ
人类β的序列。描述了-微管蛋白c[互补]DNA克隆（d . β .-1）；与人β基因序列的同源性为95.6%。-微管蛋白加工伪基因由加工mRNA的反转录衍生。该cDNA编码的氨基酸序列与猪和鸡的. β同源性较差。-微管蛋白序列之间的差异大于后者，主要差异在15个羧基末端氨基酸内。一个独立分离的，功能表达的基因组人类β。-微管蛋白序列（5 - β）与鸡和猪的- β具有高度的同源性。-这个区域的小管蛋白。人类细胞似乎含有两种不同的β。微管蛋白同形像。都是完整的。-微管蛋白cDNA克隆和仅含3”非翻译区亚克隆在人宫颈癌细胞中检测到2种mRNA；这些mRNA的长度分别为1.8和2.6千碱基，数量大致相同。两个独立的亚克隆探针构建于5. β的3”未翻译区。基因组序列还检测到2.6千碱基。微管蛋白mRNA。cDNA克隆的3”非翻译区探针与基因组序列没有交叉杂交。至少有两个人类贝塔。-微管蛋白基因在HeLa细胞中表达，每个基因指定一个不同的同型，2.6千碱基的mRNA带是至少2个配对的。微管蛋白mRNA。
The sequence of a human .beta.-tubulin c[complementary]DNA clone (D.beta.-1) is described; 95.6% homology was revealed compared with the sequence of a human .beta.-tubulin processed pseudogene derived by reverse transcription of a processed mRNA. The amino acid sequence encoded by this cDNA showed less homology with pig and chicken .beta.-tubulin sequences than the latter did to each other, with major divergence within the 15 carboxy-terminal amino acids. An independently isolated, functionally expressed genomic human .beta.-tubulin sequence (5.beta.) possessed a very high degree of homology with chicken and pig .beta.-tubulins in this region. Human cells appear to contain 2 distinct .beta.-tubulin isotypes. Both the intact .beta.-tubulin cDNA clone and a subclone containing only the 3'' untranslated region detected 2 mRNA species in HeLa [human cervical carcinoma] cells; these mRNA were 1.8 and 2.6 kilobases long and were present in about equal amounts. Two independently subcloned probes constructed from the 3'' untranslated region of the 5.beta. genomic sequence also detected a 2.6-kilobase .beta.-tubulin mRNA. The 3''-untranslated-region probes from the cDNA clone and the genomic sequence did not cross-hybridize. At least 2 human .beta.-tubulin genes, each specifying a distinct isotype, are expressed in HeLa cells, and the 2.6-kilobase mRNA band is a composite of at least 2 comigrating .beta.-tubulin mRNA.