Targeting and signaling of Rho of plants guanosine triphosphatases require synergistic interaction between guanine nucleotide inhibitor and vesicular trafficking
Targeting and signaling of Rho of plants guanosine triphosphatases require synergistic interaction between guanine nucleotide inhibitor and vesicular trafficking
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植物鸟苷三磷酸酶 Rho 的靶向和信号转导需要鸟嘌呤核苷酸抑制剂和囊泡运输之间的协同相互作用
DOI：
10.1111/jipb.12928
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发表时间：
2020-10-01
影响因子：
11.4
通讯作者：
Zhang, Yan
中科院分区：
文献类型：
--
作者：
Ge, Fu-Rong;Chai, Sen;Zhang, Yan
大多数真核细胞是极化的。调节细胞极化的常见工具箱包括 Rho 鸟苷三磷酸酶 (GTPase)，其中时空激活受到大量调节因子的调节。植物 Rho (ROP) 是植物中唯一的 Rho GTP 酶。尽管在 ROP 的调节中暗示了囊泡运输，但尚不清楚囊泡携带的 ROP 从哪里开始，是否受到动态调节，以及哪些成分参与囊泡介导的 ROP 靶向。此外，尽管 Rho 信号传导中的囊泡运输和鸟嘌呤核苷酸抑制剂 (GDI) 途径已在酵母中得到广泛研究，但尚不清楚这两种途径是否相互作用。尚不清楚它们在多细胞生物中相互作用的细胞和发育后果。在这里，我们表明 ROP 通过囊泡的动态靶向需要外壳蛋白复合物 II 和 ADP-核糖基化因子 1 介导的高尔基体后转运。囊泡携带的 ROP 在质膜和反高尔基体网络之间的运输是通过接头蛋白 1 和甾醇介导的内吞作用介导的。最后，我们发现 GDI 和囊泡运输协同调节细胞极化和 ROP 靶向，表明细胞极性的建立和维持是由进化上保守的机制调节的。
Most eukaryotic cells are polarized. Common toolbox regulating cell polarization includes Rho guanosine triphosphatases (GTPases), in which spatiotemporal activation is regulated by a plethora of regulators. Rho of plants (ROPs) are the only Rho GTPases in plants. Although vesicular trafficking was hinted in the regulation of ROPs, it was unclear where vesicle-carried ROP starts, whether it is dynamically regulated, and which components participate in vesicle-mediated ROP targeting. In addition, although vesicle trafficking and guanine nucleotide inhibitor (GDI) pathways in Rho signaling have been extensively studied in yeast, it is unknown whether the two pathways interplay. Unclear are also cellular and developmental consequences of their interaction in multicellular organisms. Here, we show that the dynamic targeting of ROP through vesicles requires coat protein complex II and ADP-ribosylation factor 1-mediated post-Golgi trafficking. Trafficking of vesicle-carried ROPs between the plasma membrane and thetrans-Golgi network is mediated through adaptor protein 1 and sterol-mediated endocytosis. Finally, we show that GDI and vesicle trafficking synergistically regulate cell polarization and ROP targeting, suggesting that the establishment and maintenance of cell polarity is regulated by an evolutionarily conserved mechanism.