Distribution of ASIC4 transcripts in the adult wild-type mouse brain.
Distribution of ASIC4 transcripts in the adult wild-type mouse brain.
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成年野生型小鼠大脑中 ASIC4 转录本的分布。
DOI：
10.1016/j.neulet.2017.03.054
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Ugawa S.
中科院分区：
文献类型：
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作者：
Hoshikawa M;Kato A;Hojo H;Shibata Y;Kumamoto N;Watanabe M;Ugawa S.
酸敏感离子通道4(ASIC4)属于神经元质子门控阳离子通道ASIC基因家族，是该家族成员中了解最少的亚型。以往对哺乳动物中枢神经系统中ASIC4表达的研究表明，ASIC4在脊髓和大脑皮层、海马体和小脑等不同脑区大量表达。然而，ASIC4转录本在哺乳动物脑中的详细分布仍有待阐明。在本研究中，用ASIC4特异的cRNA探针对野生型小鼠脑进行了放射静脉原位杂交组织化学，然后用Asic4-lacZ报告鼠Asic4tm1a(KOMP)MBP进行了X-Gal染色实验。结果发现，ASIC4在野生型脑中广泛表达，但主要集中在嗅球、梨状皮质、尾壳核、视前区、室旁核、内侧缰核、顶盖前区、外侧膝状核、杏仁复合体、上丘、脚间核和腹侧海马颗粒细胞层，这些结果与在突变脑中观察到的X-Gal阳性反应一致。此外，X-Gal染色结合免疫组织化学方法在大多数胆碱乙酰转移酶(ChAT)阳性的主神经元中发现了ASIC4转录活性的强烈信号，这些神经元位于基底前脑的胆碱能核团。我们的数据为推测ASIC4在不同大脑功能中的可能作用提供了有用的信息。
Acid-sensing ion channel 4 (ASIC4) belongs to the ASIC gene family of neuronal proton-gated cation channels, and is the least understood subtype among the members. Previous studies of ASIC4 expression in the mammalian central nervous system have shown that ASIC4 is abundantly expressed in the spinal cord and in various brain regions, such as the cerebral cortex, the hippocampus, and the cerebellum. However, the detailed distribution of ASIC4 transcripts in mammalian brains still remains to be elucidated. In the present study, radioactivein situhybridization histochemistry with an ASIC4-specific cRNA probe was performed on wild-type mouse brains, followed by X-gal staining experiments withAsic4-lacZreporter miceAsic4tm1a(KOMP)Mbp. It was found that ASIC4 mRNAs were widely expressed throughout the wild-type brain, but preferentially concentrated in the olfactory bulb, the piriform cortex, the caudate putamen, the preoptic area, the paraventricular nucleus, the medial habenular nucleus, the pretectal area, the lateral geniculate nucleus, the amygdaloid complex, the superior colliculus, the interpeduncular nucleus, and the granule cell layer of the ventral hippocampus, and these results were in agreement with the X-gal-positive reactions observed in the mutant brain. In addition, X-gal staining combined with immunohistochemistry identified intense signals for ASIC4 transcriptional activity in most of the choline acetyltransferase (ChAT)-positive principal neurons located in the basal forebrain cholinergic nuclei. Our data provide useful information to speculate possible roles of ASIC4 in diverse brain functions.