Inflammatory response to implant particulates in a macrophage/osteoblast coculture model
Inflammatory response to implant particulates in a macrophage/osteoblast coculture model
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DOI：
10.1007/s002239900145
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发表时间：
1996-11-01
影响因子：
4.2
通讯作者：
Gonzales, JB
中科院分区：
文献类型：
--
作者：
Horowitz, SM;Gonzales, JB
本研究的目的是进一步确定无菌松动时细胞对钛和聚甲基丙烯酸甲酯 (PMMA) 颗粒的反应，并确定使用帕米磷酸盐是否可以有效抑制与这种反应相关的骨吸收。巨噬细胞和成骨细胞共培养以模拟无菌松动假体周围的环境。将巨噬细胞铺在六孔板的底部，并将成骨细胞铺在培养皿插入物上，并与巨噬细胞一起放入孔中。在该系统中，巨噬细胞与 PMMA 一起孵育，导致前列腺素 E (PGE(2))、粒细胞巨噬细胞集落刺激因子 (GM-CSF) 和白细胞介素 6 (IL-6) 的释放。与钛一起孵育导致肿瘤坏死因子 (TNF) 和 IL-6 的释放。将颅盖暴露于来自暴露于 PMMA 或钛的细胞的培养基会导致钙的释放 45。将颅盖与帕米磷酸一起孵育能够抑制与暴露于巨噬细胞/成骨细胞/颗粒条件培养基相关的钙 45 的释放。植入物和骨之间界面处的骨吸收是导致骨科植入物松动的一致特征。通过抑制与植入物颗粒的炎症反应相关的骨吸收，帕米膦酸盐或其他双膦酸盐可在治疗或预防或无菌性松动方面具有临床用途。
The purpose of this study was to further define the cellular response to titanium and polymethylmethacrylate (PMMA) particles in aseptic loosening, and to determine if the use of pamidronate may be effective in inhibiting bone resorption associated with this response. Macrophages and osteoblasts were cocultured to simulate the environment around an aseptically loose prosthesis. Macrophages were plated on the bottom of six well plates and osteoblasts were plated on culture dish inserts, and placed into the wells with the macrophages. Incubation of macrophages with PMMA in this system led to release of prostaglandin E (PGE(2)), granulocyte macrophage-colony stimulating factor (GM-CSF), and interleukin-6 (IL-6). Incubation with titanium led to release of tumor necrosis factor (TNF) and IL-6. Exposure of calvaria to media from cells exposed to either PMMA or titanium led to release of calcium 45. Incubation of calvaria with pamidronate was able to inhibit release of calcium 45 associated with exposure to the macrophage/osteoblast/particle conditioned medium.Bone resorption at the interface between implant and bone is a consistent feature leading to loosening of orthopedic implants. By inhibiting bone resorption associated with the inflammatory response to implant particulates, pamidronate or other bisphosphonates may have clinical utility in the treatment or prevention or aseptic loosening.