Resistance of cell membranes to different detergents
Resistance of cell membranes to different detergents
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DOI：
10.1073/pnas.0631579100
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发表时间：
2003-05-13
影响因子：
11.1
通讯作者：
Simons, K
中科院分区：
文献类型：
--
作者：
Schuck, S;Honsho, M;Simons, K
细胞膜对温和洗涤剂溶解的部分抗性和分离的耐洗涤剂膜（DRM）的分析已被用于操作以定义膜结构域。鉴于用于此目的的洗涤剂的众多，我们试图调查是否提取不同的洗涤剂可能反映相同的基本原理域的形成。因此，我们比较了用来自两个细胞系的各种洗涤剂制备的DRM的蛋白质和脂质含量。我们发现，洗涤剂的选择性溶解膜蛋白和丰富的鞘脂和胆固醇甘油磷脂以及饱和的不饱和磷脂酰胆碱的能力有很大的不同。此外，我们观察到细胞类型依赖的变化的分子特征的DRM和特定的洗涤剂的有效性。这些结果使得不同的洗涤剂不太可能反映膜组织的相同方面，并强调了细胞膜的结构复杂性以及需要更复杂的分析工具来理解其结构。
Partial resistance of cell membranes to solubilization with mild detergents and the analysis of isolated detergent-resistant membranes (DRMs) have been used operationally to define membrane domains. Given the multitude of detergents used for this purpose, we sought to investigate whether extraction with different detergents might reflect the same underlying principle of domain formation. We therefore compared the protein and lipid content of DRMs prepared with a variety of detergents from two cell lines. We found that the detergents differ considerably in their ability to selectively solubilize membrane proteins and to enrich sphingolipids and cholesterol over glycerophospholipids as well as saturated over unsaturated phosphatidylcholine. In addition, we observed cell type-dependent variations of the molecular characteristics of DRMs and the effectiveness of particular detergents. These results make it unlikely that different detergents reflect the same aspects of membrane organization and underscore both the structural complexity of cell membranes and the need for more sophisticated analytical tools to understand their architecture.