Recognition of 5' triphosphate by RIG-I helicase requires short blunt double-stranded RNA as contained in panhandle of negative-strand virus.
Recognition of 5' triphosphate by RIG-I helicase requires short blunt double-stranded RNA as contained in panhandle of negative-strand virus.
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DOI：
10.1016/j.immuni.2009.05.008
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发表时间：
2009-07-17
期刊：
影响因子：
32.4
通讯作者：
Hartmann, Gunther
中科院分区：
文献类型：
--
作者：
Schlee, Martin;Roth, Andreas;Hornung, Veit;Hagmann, Cristina Amparo;Wimmenauer, Vera;Barchet, Winfried;Coch, Christoph;Janke, Markus;Mihailovic, Aleksandra;Wardle, Greg;Juranek, Stefan;Kato, Hiroki;Kawai, Taro;Poeck, Hendrik;Fitzgerald, Katherine A.;Takeuchi, Osamu;Akira, Shizuo;Tuschl, Thomas;Latz, Eicke;Ludwig, Janos;Hartmann, Gunther
抗病毒免疫是由对病毒核酸的免疫识别所触发的。胞质解旋酶RIG - I是病毒感染的关键感受器，它可被5′端含有三磷酸的RNA激活。激活RIG - I的RNA的确切结构仍存在争议。在此我们建立了一种用于5′三磷酸寡核糖核苷酸合成的化学方法，并发现合成的单链5′三磷酸寡核糖核苷酸无法结合并激活RIG - I。相反，添加合成的互补链可导致RIG - I的最佳结合和激活。需要带有5′三磷酸的核苷形成碱基配对的短双链构象。5′三磷酸端的3′突出端会削弱RIG - I的激活。这些结果确定了能使RIG - I完全激活的RNA结构，并解释了RIG - I如何检测负链RNA病毒，这些病毒缺乏长双链RNA，但在其单链基因组中确实含有柄环结构的平端短双链5′三磷酸RNA。
Antiviral immunity is triggered by immunorecognition of viral nucleic acids. The cytosolic helicase RIG-I is a key sensor of viral infections and is activated by RNA containing a triphosphate at the 5′end. The exact structure of RNA activating RIG-I remains controversial. Here we established a chemical approach for 5′triphosphate oligoribonucleotide synthesis and found that synthetic single-stranded 5′triphosphate oligoribonucleotides were unable to bind and activate RIG-I. Conversely, the addition of the synthetic complementary strand resulted in optimal binding and activation of RIG-I. Short double strand conformation with base pairing of the nucleoside carrying the 5′triphosphate was required. RIG-I activation was impaired by a 3′overhang at the 5′triphosphate end. These results define the structure of RNA for full RIG-I activation and explain how RIG-I detects negative strand RNA viruses which lack long double-stranded RNA but do contain panhandle blunt short double-stranded 5′triphosphate RNA in their single-stranded genome.
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影响因子：
56.9
作者：
Pichlmair, Andreas;Schulz, Oliver;Sousa, Caetano Reis E.
通讯作者：
Sousa, Caetano Reis E.
影响因子：
30.5
作者：
Latz, Eicke;Verma, Anjali;Golenbock, Douglas T.
通讯作者：
Golenbock, Douglas T.
影响因子：
64.8
作者：
Saito, Takeshi;Owen, David M.;Gale, Michael, Jr.
通讯作者：
Gale, Michael, Jr.
影响因子：
3.6
作者：
LUDWIG, J;ECKSTEIN, F
通讯作者：
ECKSTEIN, F
DOI：
10.1073/pnas.91.15.6972
发表时间：
1994-07-19
影响因子：
11.1
作者：
CAZENAVE, C;UHLENBECK, OC
通讯作者：
UHLENBECK, OC