Abrupt suspension of probiotics administration may increase host pathogen susceptibility by inducing gut dysbiosis.
Abrupt suspension of probiotics administration may increase host pathogen susceptibility by inducing gut dysbiosis.
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突然停止服用益生菌可能会导致肠道菌群失调，从而增加宿主病原体的易感性
DOI：
10.1038/srep23214
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发表时间：
2016-03-17
影响因子：
4.6
通讯作者：
Zhou Z
中科院分区：
文献类型：
--
作者：
Liu Z;Liu W;Ran C;Hu J;Zhou Z
在这项研究中，我们调查了罗非鱼益生菌管理悬浮液相关的风险，罗非鱼是一种可能模拟人类免疫受损状况的动物模型。采用添加益生菌的饲料饲喂罗非鱼14 d，然后进行为期3 d的益生菌悬浮液处理和随后的嗜水气单胞菌攻毒。出乎意料的是，益生菌株LactobacillusplantarumJCM1149的悬浮液显著地触发了宿主对A.你好我们进一步观察到，JCM 1149的悬浮液导致宿主肠道微生物群生态失调和随后的肠道代谢物（胆汁酸、氨基酸和葡萄糖）紊乱以及肠上皮损伤，引起类似于杀虫剂诱导的肠道生态失调的状况，这共同损害了罗非鱼的肠道健康和对病原体挑战的抵抗力。此外，我们确定JCM1149对罗非鱼肠粘膜的粘附相对较差，并且在悬浮后从胃肠道（GIT）快速释放，益生菌菌株的快速损失可能是肠道生态失调的直接原因。最后，其他三个低肠粘膜结合活性的益生菌乳酸杆菌菌株在给药混悬液后表现出类似的快速损失表型，并诱导更高的宿主对感染的易感性，表明风险是乳酸杆菌中的普遍现象。
In this study, we investigated the risk associated with suspension of probiotics administration in tilapia, an animal model that may mimic immune-compromised conditions in humans. Tilapias were fed for 14 days using a probiotics-supplemented diet, followed by a three-day suspension of probiotics treatment and a subsequent challenge byAeromonas hydrophila. Unexpectedly, the suspension of a probiotic strainLactobacillus plantarumJCM1149 significantly triggered susceptibility of the host toA. hydrophila. We further observed that suspension of JCM1149 resulted in host gut microbiota dysbiosis and the subsequent disorder in the intestinal metabolites (bile acids, amino acids, and glucose) and damage in the intestinal epithelium, giving rise to a condition similar to antibiotics-induced gut dysbiosis, which collectively impaired tilapia’s gut health and resistance to pathogenic challenges. Additionally, we determined that JCM1149 adhered relatively poorly to tilapia intestinal mucosa and was rapidly released from the gastrointestinal tract (GIT) after suspension, with the rapid loss of probiotic strain probably being the direct cause of gut dysbiosis. Finally, three other probioticLactobacillusstrains with low intestinal mucosa binding activity showed similar rapid loss phenotype following administration suspension, and induced higher host susceptibility to infection, indicating that the risk is a generic phenomenon inLactobacillus.