The AP-1 transcription factor homolog Pf-AP-1 activates transcription of multiple biomineral proteins and potentially participates in Pinctada fucata biomineralization.
The AP-1 transcription factor homolog Pf-AP-1 activates transcription of multiple biomineral proteins and potentially participates in Pinctada fucata biomineralization.
复制标题
AP-1 转录因子同源物 Pf-AP-1 激活多种生物矿物质蛋白的转录，并可能参与珠母贝生物矿化
DOI：
10.1038/srep14408
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发表时间：
2015-09-25
影响因子：
4.6
通讯作者：
Zhang R
中科院分区：
文献类型：
--
作者：
Zheng X;Cheng M;Xiang L;Liang J;Xie L;Zhang R
激活蛋白-1（AP-1）是一种重要的bZIP转录因子，通过特异性激活多个基因的转录来调节一系列生理过程，其在哺乳动物中的重要功能之一是参与骨矿化。我们从合浦珠母贝中分离并克隆了一个Jun/AP-1同源基因的完整cDNA，命名为Pf-AP-1。与哺乳动物相比，Pf-AP-1具有高度保守的bZIP区和磷酸化位点。组织分布分析表明Pf-AP-1在合浦藻中广泛表达，并且在外套膜中表达量极高。Pf-AP-1表达与外套膜中的多种生物矿物蛋白呈正相关。在哺乳动物细胞系中的荧光素酶报告基因测定显示，Pf-AP-1显著上调KRMP、Pearlin和Prisilkin39的启动子的转录活性。抑制Pf-AP-1的活性可抑制多种基质蛋白的表达。Pf-AP-1在贝壳再生和珍珠囊发育过程中表现出独特的表达模式，这与生物矿物蛋白的表达模式相似。这些结果表明，Pf-AP-1 AP-1同源物是一个重要的转录因子，同时调节几种生物矿物蛋白的转录，并在合浦珠母贝生物矿化中发挥作用，特别是在珍珠和贝壳形成过程中。
Activator protein-1 (AP-1) is an important bZIP transcription factor that regulates a series of physiological processes by specifically activating transcription of several genes and one of its well-chartered functions in mammals is participating in bone mineralization. We isolated and cloned the complete cDNA of a Jun/AP-1 homolog from Pinctada fucata and called it Pf-AP-1. Pf-AP-1 had a highly conserved bZIP region and phosphorylation sites compared with those from mammals. A tissue distribution analysis showed that Pf-AP-1 was ubiquitously expressed in P. fucata and the mRNA level of Pf-AP-1 is extremely high in mantle. Pf-AP-1 expression was positively associated with multiple biomineral proteins in the mantle. The luciferase reporter assay in a mammalian cell line showed that Pf-AP-1 significantly up-regulates the transcriptional activity of the promoters of KRMP, Pearlin and Prisilkin39. Inhibiting the activity of Pf-AP-1 depressed the expression of multiple matrix proteins. Pf-AP-1 showed a unique expression pattern during shell regeneration and pearl sac development, which was similar to the pattern observed for biomineral proteins. These results suggest that the Pf-AP-1 AP-1 homolog is an important transcription factor that regulates transcription of several biomineral proteins simultaneously and plays a role in P. fucata biomineralization, particularly during pearl and shell formation.