Effect of LGR4 on synovial cells and inflammatory factors in rats with traumatic osteoarthritis
Effect of LGR4 on synovial cells and inflammatory factors in rats with traumatic osteoarthritis
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DOI：
10.26355/eurrev_201908_18716
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发表时间：
2019-08-01
影响因子：
3.3
通讯作者：
Wu, X-Z
中科院分区：
文献类型：
--
作者：
Ge, J-J;Lu, W-K;Wu, X-Z
目的：创伤性关节炎是骨科最常见的疾病之一。LGR4参与骨形成和骨发育。然而，LGR4在创伤性骨关节炎大鼠滑膜细胞中的作用尚未见报道。材料与方法：SD大鼠随机分为对照组和模型组。实时定量聚合酶链式反应(RT-PCR)。用Western印迹和酶联免疫吸附试验(ELISA)分析LGR4在滑膜组织和滑液中的表达。分离培养滑膜细胞，将LGR4-pcDNA3.1载体转入细胞内。用四甲基偶氮唑盐比色法和EDU比色法检测细胞增殖，用Caspase-3活性试剂盒检测Caspase-3活性。用酶联免疫吸附试验检测炎症因子白介素1、肿瘤坏死因子α、白介素6的分泌。Western blot法检测核因子-kappaB信号通路的变化。结果：模型组。滑膜组织中LGR4基因表达明显减少，滑液中LGR4的分泌量明显减少(P<0.05)。
OBJECTIVE: Traumatic arthritis is one of the most common diseases in orthopedics. LGR4 is involved in bone formation and bone development. However, the role of LGR4 in synovial cells of rats with traumatic osteoarthritis has not been reported.MATERIALS AND METHODS: Sprague Dawley (SD) rats were randomly divided into the control group and model group. The Real Time-Polymerase Chain Reaction (RT-PCR). Western blot, and Enzyme-Linked lmmunosorbent Assay (ELISA) were used to analyze the expression of LGR4 in synovial tissue and synovial fluid. Synovial cells were isolated and cultured, followed by transfection of LGR4-pcDNA3.1 plasmid into cells. Cell proliferation was analyzed by MTT and EdU assay, and the Caspase-3 activity was assessed using the Caspase-3 activity kit. The secretion of the inflammatory factors interleukin-1 (IL-1), tumor necrosis factor-alpha (TNF-alpha), and interleukin-6 (IL-6) was detected by ELISA. NF-kappa B signaling pathway changes were evaluated by the Western blot.RESULTS: In the model group. LGR4 mRNA expression in synovial tissue was significantly decreased, and the secretion of LGR4 in the synovial fluid was significantly decreased compared with the control group (p