Transcriptional derepression of the Saccharomyces cerevisiae HSP26 gene during heat shock.
Transcriptional derepression of the Saccharomyces cerevisiae HSP26 gene during heat shock.
复制标题
热休克期间酿酒酵母 HSP26 基因的转录去抑制。
DOI：
10.1128/mcb.10.12.6362-6373.1990
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发表时间：
1990
影响因子：
5.3
通讯作者：
Lindquist,S
中科院分区：
文献类型：
--
作者：
Susek,RE;Lindquist,S
热休克蛋白26是酿酒酵母中的一种小分子热休克蛋白，在高温和其他类型的应激反应中积累。在正常发育过程中，当细胞进入生长稳定期或开始形成孢子时，它也会积累（S. Kurtz，J. Rossi，L. Petko和S. Lindquist，Science 231：1154-1157，1986）。缺失和插入突变分析表明转录调控在调控HSP 26表达中起着关键作用。发现HSP 26启动子是复杂的，似乎包含抑制元件以及激活元件。几个上游缺失突变导致HSP 26的强组成性表达。此外，HSP 26基因的上游序列抑制了异源热休克基因的组成型表达。我们认为基础阻遏和热诱导的转录抑制在调节HSP 26的表达中起主要作用。我们分析的重组构建体中没有一个将负责热休克调控的顺式调控序列与负责HSP 26发育调控的顺式调控序列分开。抑制HSP 26的转录可能是HSP 26在酵母细胞中诱导表达的一般机制。这种调控机制与大多数其他热休克基因的调控机制非常不同。
hsp26, the small heat shock protein ofSaccharomyces cerevisiae, accumulates in response to heat and other types of stress. It also accumulates during the normal course of development, as cells enter stationary phase growth or begin to sporulate (S. Kurtz, J. Rossi, L. Petko, and S. Lindquist, Science 231:1154-1157, 1986). Analysis of deletion and insertion mutations demonstrated that transcriptional control plays a critical role in regulatingHSP26expression. TheHSP26promoter was found to be complex and appears to contain repressing elements as well as activating elements. Several upstream deletion mutations resulted in strong constitutive expression ofHSP26. Furthermore, upstream sequences from theHSP26gene repressed the constitutive expression of a heterologous heat shock gene. We propose that basal repression and heat-induced depression of transcription play major roles in regulating the expression ofHSP26. None of the recombinant constructs that we analyzed separated cis-regulatory sequences responsible for heat shock regulation from those responsible for developmental regulation ofHSP26. Depression ofHSP26transcription may be the general mechanism ofHSP26induction in yeast cells. This regulatory scheme is very different from that described for the regulation of most other heat shock genes.