Proinflammatory cytokines differentially regulate hyaluronan synthase isoforms in fetal and adult fibroblasts
Proinflammatory cytokines differentially regulate hyaluronan synthase isoforms in fetal and adult fibroblasts
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DOI：
10.1053/jpsu.2000.6869
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发表时间：
2000-06-01
影响因子：
2.4
通讯作者：
Yager, DR
中科院分区：
文献类型：
--
作者：
Kennedy, CI;Diegelmann, RF;Yager, DR
背景/目的：胎儿伤口愈合是在富含透明质酸的环境中发生的相对无瘢痕的过程。了解透明质酸表达的调节可能提供对胎儿修复过程的深入了解。因此，本研究的目的是比较促炎细胞因子白细胞介素-1 β对透明质酸和透明质酸合成酶转录的调节作用（IL-1 β）和肿瘤坏死因子-α方法：沉积在未处理的成纤维细胞或用IL-1 β或TNF-α处理的成纤维细胞的培养基中的海洛宁通过使用碘I 125- 125的测定来测定。透明质酸结合蛋白。HAS转录水平进行了比较，在使用核糖核酸酶保护assay.Results：IL-1 β诱导的胎儿和成人成纤维细胞的透明质酸积累的增加。相反，TNF-α仅在胎儿成纤维细胞中诱导更高水平的透明质酸。胎儿和成人成纤维细胞组成型表达HAS-2和HAS-3转录水平。促炎细胞因子诱导的差异增加HAS-1和HAS-3的转录水平。结论：观察到差异调节透明质酸的积累和胎儿和成人皮肤成纤维细胞的HAS转录水平。胎儿成纤维细胞对细胞因子的微弱反应可能与胎儿修复相关的最小炎症有关。Copyright（C）2000 by W.B.桑德斯公司
Background/Purpose: Fetal wound healing is a relatively scarless process that occurs in an hyaluronan-rich environment. Understanding the regulation of hyaluronan expression may provide insight into the process of fetal repair. Therefore, the purpose of this study was to compare the regulation of hyaluronan and hyaluronan synthase transcripts by the proinflammatory cytokines interleukin-1 beta (IL-1 beta) and tumor necrosis factor-alpha (TNF-alpha) in human adult and fetal fibroblasts.Methods: Hyaluronan deposited in the medium of untreated fibroblasts or fibroblasts treated with either IL-1 beta or TNF-alpha was determined by an assay utilizing iodine I 125-hyaluronan binding protein. HAS transcript levels were compared in using a ribonuclease protection assay.Results: IL-1 beta induced an increase in hyaluronan accumulation by both fetal and adult fibroblasts. in contrast, TNF-alpha induced higher levels of hyaluronan only in fetal fibroblasts. HAS-2 and HAS-3 transcript levels were constitutively expressed by both fetal and adult fibroblasts. Proinflammatory cytokines induced a differential increase in HAS-1 and HAS-3 transcript levels.Conclusions: Differential regulation was observed in hyaluronan accumulation and for HAS transcript levels in fetal and adult dermal fibroblasts. The muted response of fetal fibroblasts to cytokines may be relevant to the minimal inflammation associated with fetal repair. Copyright (C) 2000 by W.B. Saunders Company.