Sperm DNA oxidative damage and DNA adducts.
Sperm DNA oxidative damage and DNA adducts.
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DOI：
10.1016/j.mrgentox.2015.09.002
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发表时间：
2015-12
期刊：
影响因子：
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通讯作者：
Lin WY
中科院分区：
文献类型：
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作者：
Jeng HA;Pan CH;Chao MR;Lin WY
本研究的目的是探讨焦炉工人接触多环芳烃后精子的DNA损伤和加合物。在精子发生过程中重复测量进行了纵向研究。焦炉工人（n=112）从焦炉厂为PAH暴露组，而管理员和安全人员（n=67）为对照组。同时分析常规精液参数（浓度、活力、活力和形态）;精子DNA完整性终点的评估包括DNA片段化、大体积DNA加合物和8-氧代-7，8-二氢-2 ′-脱氧鸟苷（8-oxo-dGuo）。采用末端脱氧核苷酸转移酶介导的dUTP缺口末端标记法（TUNEL）和精子染色质结构分析法（SCSA）测定精子DNA片段化程度。与对照组相比，PAH暴露组的大体积DNA加合物和8-oxo-dGuo显著增加（分别为Ps = 0.002和0.045）。焦炉工PAH暴露组DNA断裂率和变性率与对照组比较差异均无统计学意义（Ps分别为0.232和0.245）。常规精液参数和DNA完整性终点不相关。8-oxo-dGuo的浓度与通过TUNEL和SCSA测量的DNA片段化百分比正相关（分别为Ps = 0.045和0.034）。然而，8-oxo-dGuo的浓度和DNA片段化的百分比与大体积DNA加合物的浓度无关。总之，长期暴露于多环芳烃的焦炉工人精子DNA完整性降低。氧化应激可导致DNA片段化的程度。大体积DNA加合物可能与精子中DNA片段化和氧化加合物的形成无关。建议将监测精子DNA完整性作为评估职业和环境毒素对精子影响过程的一部分。
The objective of this study was to investigate DNA damage and adducts in sperm from coke oven workers who have been exposed to polycyclic aromatic hydrocarbons. A longitudinal study was conducted with repeated measurements during spermatogenesis. Coke-oven workers (n=112) from a coke-oven plant served the PAH-exposed group, while administrators and security personnel (n=67) served the control. Routine semen parameters (concentration, motility, vitality, and morphology) were analyzed simultaneously; the assessment of sperm DNA integrity endpoints included DNA fragmentation, bulky DNA adducts, and 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxo-dGuo). The degree of sperm DNA fragmentation was measured using the terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) assay and sperm chromatin structure assay (SCSA). The PAH-exposed group had a significant increase in bulky DNA adducts and 8-oxo-dGuo compared to the control subjects (Ps = 0.002 and 0.045, respectively). Coke oven workers' percentages of DNA fragmentation and denaturation from the PAH-exposed group were not significantly different from those of the control subjects (Ps = 0.232 and 0.245, respectively). Routine semen parameters and DNA integrity endpoints were not correlated. Concentrations of 8-oxo-dGuo were positively correlated with percentages of DNA fragmentation measured by both TUNEL and SCSA (Ps = 0.045 and 0.034, respectively). However, the concentrations of 8-oxo-dGuo and percentages of DNA fragmentation did not correlate with concentrations of bulky DNA adducts. In summary, coke oven workers with chronic exposure to PAHs experienced decreased sperm DNA integrity. Oxidative stress could contribute to the degree of DNA fragmentation. Bulky DNA adducts may be independent of the formation of DNA fragmentation and oxidative adducts in sperm. Monitoring sperm DNA integrity is recommended as a part of the process of assessing the impact of occupational and environmental toxins on sperm.