Paenibacillus taihuensis sp. nov., isolated from an eutrophic lake.
Paenibacillus taihuensis sp. nov., isolated from an eutrophic lake.
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DOI：
10.1099/ijs.0.050419-0
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发表时间：
2013-10
影响因子：
2.8
通讯作者：
Yu-fan Wu;Qing-long Wu;Shuangjiang Liu
中科院分区：
文献类型：
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作者：
Yu-fan Wu;Qing-long Wu;Shuangjiang Liu
从分解的藻渣中分离到革兰氏染色阴性、兼性厌氧和内孢子形成的杆状细菌THMBG22(T)和R24。16S rRNA基因序列的系统发育分析表明，两株菌株亲缘关系较近（相似度为99.7%），与sacheonensis Paenibacillus DSM 23054(T)（97- 97.1%）和phyllosphaerae Paenibacillus DSM 17399(T)（96.1- 96.4%）亲缘关系也较近。基于rpob的系统发育分析也支持这种联系。在20-40°C（最适30-37°C）和pH 5.0-9.0（最适pH 6.0-7.0）条件下观察生长。细胞中唯一的呼吸醌为MK-7，主要的细胞脂肪酸为antiiso - c15: 0。它们的细胞极性脂质由磷脂酰甘油、二磷脂酰甘油、磷脂酰乙醇胺和12种未识别的极性脂质组成。其细胞壁肽聚糖的二氨基为中二氨基亚苯甲酸。THMBG22(T)与R24的DNA-DNA杂交值为84%，与有效已发表的亲缘关系最密切的种（P. sacheonensis）的DNA-DNA亲缘度为35- 37%。这些结果支持将新分离物归为类芽孢杆菌属，并将它们与先前描述的类芽孢杆菌区分开来。因此，我们认为菌株THMBG22(T)和R24代表了Paenibacillus属的一个新种，命名为Paenibacillus taihuensis sp. nov.，菌株类型为THMBG22(T) (= CGMCC 1.10966（T) = NBRC 108766(T)）。
Two Gram-stain-negative, facultatively anaerobic and endospore-forming rod-shaped bacterial strains, THMBG22(T) and R24, were isolated from decomposing algal scum. Phylogenetic analysis of 16S rRNA gene sequences showed that the two strains were closely related to each other (99.7 % similarity) and that they were also closely related to Paenibacillus sacheonensis DSM 23054(T) (97-97.1 %) and Paenibacillus phyllosphaerae DSM 17399(T) (96.1-96.4 %). This affiliation was also supported by rpoB-based phylogenetic analyses. Growth was observed at 20-40 °C (optimum, 30-37 °C) and at pH 5.0-9.0 (optimum, pH 6.0-7.0). The cells contained MK-7 as the sole respiratory quinone and anteiso-C15 : 0 as the major cellular fatty acid. Their cellular polar lipids were composed of phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and 12 unidentified polar lipids. The diamino acid of their cell-wall peptidoglycan was meso-diaminopimelic acid. The DNA-DNA hybridization value between THMBG22(T) and R24 was 84 %, and DNA-DNA relatedness to the most closely related species with a validly published name (P. sacheonensis) was 35-37 %. These results supported the assignment of the new isolates to the genus Paenibacillus and also distinguished them from the previously described species of the genus Paenibacillus. Hence, it is proposed that strains THMBG22(T) and R24 represent a novel species of the genus Paenibacillus, with the name Paenibacillus taihuensis sp. nov. The type strain is THMBG22(T) ( = CGMCC 1.10966(T) = NBRC 108766(T)).