THE PURIFICATION AND CHARACTERIZATION OF FETAL LIVER HEMATOPOIETIC STEM-CELLS
THE PURIFICATION AND CHARACTERIZATION OF FETAL LIVER HEMATOPOIETIC STEM-CELLS
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DOI：
10.1073/pnas.92.22.10302
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发表时间：
1995-10-24
影响因子：
11.1
通讯作者：
WEISSMAN, IL
中科院分区：
文献类型：
--
作者：
MORRISON, SJ;HEMMATI, HD;WEISSMAN, IL
Thy-1（lo）Sca-1（+）Lin（-）Mac-1（+）CD 4（-）细胞已从C57 BL-Thy-1.1胎儿的肝脏中分离。该群体似乎是基本上纯的造血干细胞（HSC）群体，因为仅将6个细胞注射到致死性辐照的成年受体中产生供体细胞重建小鼠的有限稀释频率。在这个群体中，67%至77%的克隆表现出长期的多谱系祖细胞活性。这个群体似乎包括胎儿肝脏中所有的长期多谱系重建祖细胞。高比例的细胞处于周期中，并且该群体中的细胞的绝对数量在胎肝中每天加倍，直到产后14.5天。在交配后15.5天，该种群的频率显著下降福尔斯。长期重建HSC克隆从胎肝引起更高水平的重建在致死照射小鼠比长期重建HSC从骨髓。HSC的确切表型和功能特征根据个体发育过程中的组织和时间而变化。
Thy-1(lo)Sca-1(+)Lin(-)Mac-1(+)CD4(-) cells have been isolated from the livers of C57BL-Thy-1.1 fetuses. This population appears to be an essentially pure population of hematopoietic stem cells (HSC), in that injection of only six cells into lethally irradiated adult recipients yields a limit dilution frequency of donor cell-reconstituted mice. Sixty-seven to 77% of clones in this population exhibit long-term multilineage progenitor activity. This population appears to include all long-term multilineage reconstituting progenitors in the fetal liver. A high proportion of cells are in cycle, and the absolute number of cells in this population doubles daily in the fetal liver until 14.5 days postcoitum. At 15.5 days post-coitum, the frequency of this population falls dramatically. Long-term reconstituting HSC clones from the fetal liver give rise to higher levels of reconstitution in lethally irradiated mice than long-term reconstituting HSC from the bone marrow. The precise phenotypic and functional characteristics of HSC vary according to tissue and time during ontogeny.