The gene csd is the primary signal for sexual development in the honeybee and encodes an SR-type protein
The gene csd is the primary signal for sexual development in the honeybee and encodes an SR-type protein
复制标题
DOI：
10.1016/s0092-8674(03)00606-8
复制
发表时间：
2003-08-22
期刊：
影响因子：
64.5
通讯作者：
Omholt, SW
中科院分区：
文献类型：
--
作者：
Beye, M;Hasselmann, M;Omholt, SW
单倍体生物约占动物总数的20%。雄性是由未受精卵发育而来，而雌性是由受精卵发育而来。然而，性别决定的潜在机制似乎是多种多样的，人们对其了解甚少。我们解剖了蜜蜂的互补性决定基因（csd）位点，以了解其分子基础。在这个物种中，csd作为主要的性别决定信号，在种群中有几个等位基因分离。雄性是半合子，雌性是杂合子；当基因座是纯合子时，就会产生不能繁殖的二倍体雄性。我们用位置克隆和抑制分析对csd进行了表征。CSD等位基因是高度可变的，没有发现性别间的转录差异。这些结果表明，csd是通过其等位基因组成支配性发育的主要信号。双翅目昆虫的csd与基因的结构相似性提示了一些功能关系，否则似乎是不相关的性别决定机制。
Haplodiploid organisms comprise about 20% of animals. Males develop from unfertilized eggs while females are derived from fertilized eggs. The underlying mechanisms of sex determination, however, appear to be diverse and are poorly understood. We have dissected the complementary sex determiner (csd) locus in the honeybee to understand its molecular basis. In this species, csd acts as the primary sex-determining signal with several alleles segregating in populations. Males are hemizygous and females are heterozygous at this locus; nonreproducing diploid males occur when the locus is homozygous. We have characterized csd by positional cloning and repression analysis. csd alleles are highly variable and no transcription differences were found between sexes. These results establish csd as a primary signal that governs sexual development by its allelic composition. Structural similarity of csd with tra genes of Dipteran insects suggests some functional relation of what would otherwise appear to be unrelated sex-determination mechanisms.