Elimination of POR expression correlates with red leaf formation in Amaranthus tricolor.
Elimination of POR expression correlates with red leaf formation in Amaranthus tricolor.
复制标题
POR 表达的消除与三色苋中红叶的形成相关。
DOI：
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发表时间：
2001
期刊：
影响因子：
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通讯作者：
M. Sugiyama
中科院分区：
文献类型：
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作者：
K. Iwamoto;H. Fukuda;M. Sugiyama
三色苋L.tricolor cv.早期壮丽是一种观赏性的紫红色植物，在夏末到初秋期间会长出红色的叶子，而不是绿色的叶子。红叶形成在短日照条件下有促进作用，夜间处理则有延迟作用。红叶的特征是较低水平的叶绿素积累，而不是较高水平的红色素(甜菜素)积累。然而，在红叶和绿叶中都检测到了生成镁原卟啉的代谢活性，镁原卟啉是叶绿素生物合成途径的中间产物。通过RNA凝胶印迹分析，分析了9个编码8种参与叶绿素生物合成的酶的基因的表达。在这些酶中，只有NADPH-原叶绿素氧化还原酶(POR)的基因表达有红叶专一性降低，POR是催化后期叶绿素生物合成的关键酶。此外，免疫印迹分析表明，在红叶中没有POR蛋白(S)的积累。这些数据表明，POR基因表达的抑制以及由此导致的叶绿素合成活性的丧失在三色沙打旺红叶的形成中起着一定的作用。
Amaranthus tricolor L. tricolor cv. Earlysplendor, an ornamental amaranth, generates red leaves instead of green leaves in late summer to early autumn. Red leaf formation was promoted under short-day conditions and delayed by night-break treatments. Red leaves were characterized by lower levels of chlorophyll accumulation rather than higher levels of red pigment (betacyanin) accumulation. However, the metabolic activity toward the production of Mg-protoporphyrin, an intermediate in the biosynthesis pathway for chlorophyll, was detected in red leaves as well as in green leaves. RNA gel blot analysis was performed to assess the expression of nine genes encoding eight enzymes involved in chlorophyll biosynthesis. Among these enzymes, red-leaf-specific reduction of gene expression was observed only for NADPH-protochlorophyllide oxidoreductase (POR), a key enzyme catalyzing a later step of chlorophyll biosynthesis. In addition, immunoblot analysis showed no accumulation of POR protein(s) in red leaves. These data indicate that the repression of POR gene expression and resultant loss of chlorophyll synthesis activity plays a role in red leaf formation of A. tricolor.
影响因子：
11.6
作者：
BOWMAN, JL;SMYTH, DR;MEYEROWITZ, EM
通讯作者：
MEYEROWITZ, EM