Molecular and phylogenetic characterization of a novel putative membrane transporter (SLC10A7), conserved in vertebrates and bacteria
Molecular and phylogenetic characterization of a novel putative membrane transporter (SLC10A7), conserved in vertebrates and bacteria
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DOI：
10.1016/j.ejcb.2007.06.001
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发表时间：
2007-08-01
影响因子：
6.6
通讯作者：
Geyer, Joachim
中科院分区：
文献类型：
--
作者：
Godoy, Jose R.;Fernandes, Carla;Geyer, Joachim
“溶质载体家族SLC 10”由六个注释的人类成员组成，包括两个胆汁酸载体（SLC 10A 1和SLC 10A 2），一个类固醇硫酸盐转运蛋白（SLC 10A 6）和三个孤儿载体（SLC 10A 3至SLC 10A 5）。在这项研究中，我们报告了SLC 10家族的一个新成员SLC 10A 7（以前称为C4 orf 13）的分子特征和表达分析。SLC 10A 7蛋白在人、小鼠、大鼠中由340-343个氨基酸组成。和蛙的氨基酸序列，并且显示出> 85%的总体氨基酸序列同一性。SLC 10A 7基因包含12个编码外显子，并显示广泛的组织表达模式。当在非洲爪蟾卵母细胞和HEK 293细胞中表达时，在质膜中检测到SLC 10A 7，但没有显示对胆汁酸和类固醇硫酸盐的转运活性。通过对HEK 293细胞中的双重血凝素（HA）和FLAG标记的SLC 10A 7蛋白的免疫荧光分析，我们建立了10个跨膜结构域的拓扑结构，其N-末端和C-末端具有细胞内顺式取向。这种拓扑结构模式明显不同于其他SLC 10成员的七跨膜结构域拓扑结构，但类似于迄今未表征的非脊椎动物SLC 10A 7相关蛋白。与仅限于脊椎动物的分类学分支的已建立的SLC 10成员相反，SLC 10 A7相关蛋白也存在于酵母、植物和细菌中，使得SLC 10 A7在分类学上成为该载体家族中最广泛的成员。脊椎动物和细菌SLC 10A 7蛋白表现出>20%的序列同一性，这高于SLC 10A 7与SLC 10载体家族的任何其他成员的序列同一性。(c)2007年，Elsevier GmbH。All rights reserved.
The 'Solute Carrier Family SLC10' consists of six annotated members in humans, comprising two bile acid carriers (SLC10A1 and SLC10A2), one steroid sulfate transporter (SLC10A6), and three orphan carriers (SLC10A3 to SLC10A5). In this study we report molecular characterization and expression analysis of a novel member of the SLC10 family, SLC10A7, previously known as C4orf13. SLC10A7 proteins consist of 340-343 amino acids in humans, mice, rats. and frogs and show an overall amino acid sequence identity of >85%. SLC10A7 genes comprise 12 coding exons and show broad tissue expression pattern. When expressed in Xenopus laevis oocytes and HEK293 cells, SLC10A7 was detected in the plasma membrane but revealed no transport activity for bile acids and steroid sulfates. By immunofluorescence analysis of dual hemagglutinin (HA)- and FLAG-labeled SLC10A7 proteins in HEK293 cells, we established a topology of 10 transmembrane domains with an intracellular cis orientation of the N-terminal and C-terminal ends. This topology pattern is clearly different from the seven-transmembrane domain topology of the other SLC10 members but similar to hitherto uncharacterized non-vertebrate SLC10A7-related proteins. In contrast to the established SLC10 members, which are restricted to the taxonomic branch of vertebrates, SLC10A7-related proteins exist also yeasts, plants, and bacteria, making SLC10A7 taxonomically the most widespread member of this carrier family. Vertebrate and bacterial SLC10A7 proteins exhibit >20% sequence identity, which is higher than the sequence identity of SLC10A7 to any other member of the SLC10 carrier family. (c) 2007 Elsevier GmbH. All rights reserved.