Gata2 provides an early anterior bias and uncovers a global positioning system for polarity in the amniote embryo.
Gata2 provides an early anterior bias and uncovers a global positioning system for polarity in the amniote embryo.
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DOI：
10.1242/dev.081901
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发表时间：
2012-11
期刊：
影响因子：
--
通讯作者：
Stern CD
中科院分区：
文献类型：
--
作者：
Bertocchini F;Stern CD
在脊椎动物发育过程中，第一个被指定的轴是介于原肠胚形成开始的位置和胚胎的相反极之间的轴（两栖动物和鱼类的背-腹轴，羊膜动物的前后轴）。这依赖于节点活动，但不同的脊椎动物在这种活动的位置上是不同的。在小鸡中，已知最早的不对称是tgf β相关因子Vg1的后向表达，接近未来的节点表达域。这里我们发现转录因子GATA2在这个阶段之前表达。GATA2影响原始条纹形成的位置，其作用独立于Vg1和Wnt，且位于其上游。然而，虽然Vg1是条纹形成所必需的，但GATA2并不能作为绝对的前方向指示物，而是提供了前偏置。这些发现指出了以前未被怀疑的早期羊膜胚胎极性的全局决定因素。
The first axis to be specified during vertebrate development is that between the site where gastrulation will begin and the opposite pole of the embryo (dorso-ventral axis in amphibians and fish, anterior-posterior in amniotes). This relies on Nodal activity, but different vertebrates differ in how this activity is positioned. In chick, the earliest known asymmetry is posterior expression of the TGFβ-related factor Vg1, close to the future Nodal expression domain. Here we show that the transcription factor GATA2 is expressed anteriorly before this stage. GATA2 influences the site of primitive streak formation and its role is independent from, and upstream of, Vg1 and Wnt. However while Vg1 is required for streak formation, GATA2 does not act as an absolute anterior specifier, but provides an anterior bias. These findings point to previously unsuspected global determinants of polarity of the early amniote embryo.