Fuzhuan brick tea polysaccharides serve as a promising candidate for remodeling the gut microbiota from colitis subjects in vitro: Fermentation characteristic and anti-inflammatory activity
Fuzhuan brick tea polysaccharides serve as a promising candidate for remodeling the gut microbiota from colitis subjects in vitro: Fermentation characteristic and anti-inflammatory activity
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DOI：
10.1016/j.foodchem.2022.133203
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发表时间：
2022-05-19
期刊：
影响因子：
8.8
通讯作者：
Liu, Zhonghua
中科院分区：
文献类型：
--
作者：
Chen, Guijie;Wang, Mingjia;Liu, Zhonghua
茯苓砖茶多糖纯化组分3（FBTPS-3）具有减轻结肠炎和调节肠道菌群的作用。然而，FBTPS-3的抗炎作用与肠道微生物群之间的关系仍然未知。因此，采用体外厌氧发酵的方法来研究其可能的机制。FBTPS-3可以被来自炎症性肠病（IBD）受试者的肠道微生物群利用和降解。此外，FBTPS-3还能调节IBD肠道菌群的组成和结构，使其接近健康组。FBTPS-3通过增加拟杆菌和减少大肠杆菌/志贺氏菌对IBD肠道微生物群显示出上级调节作用。此外，发酵液而不是FBTPS-3本身对脂多糖处理的RAW 264. 7巨噬细胞表现出抗炎作用，这可能是由于代谢产物如短链脂肪酸（SCFA）。因此，FBTPS-3有望成为通过调节肠道菌群和促进SCFAs产生来治疗IBD的新型益生元。
The purified fraction 3 of polysaccharides from Fuzhuan brick tea (FBTPS-3) could attenuate the colitis and modulate the gut microbiota. However, the relationship between anti-inflammatory effect of FBTPS-3 and the gut microbiota is still unknown. Thus, the anaerobic fermentation in vitro was used to investigate the potential mechanism. FBTPS-3 could be utilized and degraded by gut microbiota from inflammatory bowel disease (IBD) subjects. Furthermore, FBTPS-3 could modulate the composition and structure of IBD gut microbiota toward to that of healthy group. FBTPS-3 showed a superior modulated effect on IBD gut microbiota by increasing Bacteroides and decreasing Escherichia/Shigella. Furthermore, the fermentation solution rather than FBTPS-3 itself showed anti-inflammatory effects on lipopolysaccharide-treated RAW264.7 macrophages, which might be due to the metabolites such as short-chain fatty acids (SCFAs). Thus, FBTPS-3 can be expected as novel prebiotics for treatment of IBD via modulating gut microbiota, and promoting the production of SCFAs.