Proteomic analysis reveals that COP9 signalosome complex subunit 7A (CSN7A) is essential for the phase transition of migratory locust.
Proteomic analysis reveals that COP9 signalosome complex subunit 7A (CSN7A) is essential for the phase transition of migratory locust.
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蛋白质组学分析表明 COP9 信号体复合体亚基 7A (CSN7A) 对于飞蝗的相变至关重要
DOI：
10.1038/srep12542
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发表时间：
2015-07-27
影响因子：
4.6
通讯作者：
Kang L
中科院分区：
文献类型：
--
作者：
Tong XW;Chen B;Huang LH;Feng QL;Kang L
飞蝗表现出一个可逆的，密度依赖的两个阶段之间的聚集和独居的过渡。这种现象是一种典型的行为可塑性。本文报道了COP9信号体复合物亚基7A（CSN7A）参与蝗虫相变的调控。首先，通过定量蛋白质组学分析，鉴定了90个在两个阶段之间差异表达的蛋白质。与结构形成、黑化和能量代谢相关的蛋白质含量较高，而与消化、吸收和化学传感相关的蛋白质含量较高。随后，发现包括CSN7A在内的10种蛋白质揭示了两个阶段之间的差异mRNA表达谱。CSN7A基因在蜂群中的表达量高于孤养体，但在32 h的隔离过程中，蜂群中的CSN7A基因表达量显著下降。而在拥挤饲养过程中，孤雌虫体内的mRNA水平基本保持不变。最后，也是重要的是，RNA干扰CSN7A在gregaria中导致了明显的相变，向孤立在24 h内。这表明CSN7A在飞蝗从群居到独居的转变过程中起着重要的作用。据我们所知，这是首次报道CSN在动物行为可塑性中的作用。
The migratory locust displays a reversible, density-dependent transition between the two phases of gregaria and solitaria. This phenomenon is a typical kind of behavior plasticity. Here, we report thatCOP9 signalosome complex subunit 7A(CSN7A) is involved in the regulation of locust phase transition. Firstly, 90 proteins were identified to express differentially between the two phases by quantitative proteomic analysis. Gregaria revealed higher levels in proteins related to structure formation, melanism and energy metabolism, whereas solitaria had more abundant proteins related to digestion, absorption and chemical sensing. Subsequently, ten proteins including CSN7A were found to reveal differential mRNA expression profiles between the two phases. TheCSN7Ahad higher mRNA level in the gregaria as compared with the solitaria and the mRNA amount in the gregaria decreased remarkably during the 32 h-isolation. However, the mRNA level in the solitaria kept constant during the crowding rearing. Finally and importantly, RNA interference ofCSN7Ain gregaria resulted in obvious phase transition towards solitaria within 24 h. It suggests thatCSN7Aplays an essential role in the transition of gregaria towards solitaria in the migratory locust. To our knowledge, it’s the first time to report the role of CSN in behavior plasticity of animals.