A complete BAC-based physical map of the Arabidopsis thaliana genome
A complete BAC-based physical map of the Arabidopsis thaliana genome
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DOI：
10.1038/10334
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发表时间：
1999-07-01
期刊：
影响因子：
30.8
通讯作者：
Altmann, T
中科院分区：
文献类型：
--
作者：
Mozo, T;Dewar, K;Altmann, T
拟南芥（Arabidopsis thaliana）是一种小型开花植物，是植物分子遗传学的主要模式系统。许多科学家的努力已经制作出了基因图谱，提供了广泛的基因组覆盖范围（http：//genome-www.stanford.edulopsis/maps.html）。最近，已经建立了详细的基于YAC、BAG、P1和粘粒的物理图谱（即，将基因组区域表示为相应文库的重叠克隆的集合），其延伸到从数百个内切酶（2，3）到整个染色体（4-9）的广泛基因组区域。这些地图提供了一个入口，以获得更深入的了解A。拟南芥基因组结构A.已经选择了thaliana作为第一个大规模计划的主题，该计划旨在确定植物的全基因组序列（10）。这个测序项目，以及对基于图位的基因克隆的日益增长的兴趣，突出了对这种植物物种的完整和准确的物理图的需求。为了给科学界提供高质量的资源，我们在这里提供了一个完整的物理地图A。该图谱由27个重叠群组成，覆盖整个基因组，除了假定的着丝粒区、核仁组织区（NOR）和端粒区。这是第一个报道的完全基于BAC克隆的复杂生物体的图谱，它代表了迄今为止为任何植物基因组建立的最同质和最完整的物理图谱。此外，这里进行的分析作为一个有效的物理映射程序使用BAC克隆，可应用于其他复杂的基因组的模型。
Arabidopsis thaliana is a small flowering plant that serves as the major model system in plant molecular genetics'. The efforts of many scientists have produced genetic maps that provide extensive coverage of the genome (http://genome-www.stanford.edulArabidopsis/maps.html). Recently, detailed YAC, BAG, P1 and cosmid-based physical maps (that is, representations of genomic regions as sets of overlapping clones of corresponding libraries) have been established that extend over wide genomic areas ranging from several hundreds of kilobases(2,3) to entire chromosome(4-9). These maps provide an entry to gain deeper insight into the A. thaliana genome structure. A. thaliana has been chosen as the subject of the first large-scale project intended to determine the full genome sequence of a plant(10). This sequencing project, together with the increasing interest in map-based gene cloning, has highlighted the requirement for a complete and accurate physical map of this plant species. To supply the scientific community with a high-quality resource, we present here a complete physical map of A. thaliana using essentially the IGF BAC library(11) The map consists of 27 contigs that cover the entire genome, except for the presumptive centromeric regions, nucleolar organization regions (NOR) and telomeric areas. This is the first reported map of a complex organism based entirely on BAC clones and it represents the most homogeneous and complete physical map established to date for any plant genome. Furthermore, the analysis performed here serves as a model for an efficient physical mapping procedure using BAC clones that can be applied to other complex genomes.