CCA1 and LHY contribute to nonhost resistance to Pyricularia oryzae (syn. Magnaporthe oryzae) in Arabidopsis thaliana.
CCA1 and LHY contribute to nonhost resistance to Pyricularia oryzae (syn. Magnaporthe oryzae) in Arabidopsis thaliana.
复制标题
CCA1 和 LHY 有助于拟南芥中对稻瘟病菌（同名 Magnaporthe oryzae）的非宿主抗性。
DOI：
10.1080/09168451.2019.1660612
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发表时间：
2020
期刊：
影响因子：
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通讯作者：
Ishikawa A.
中科院分区：
文献类型：
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作者：
Yamaura S;Yamauchi Y;Makihara M;Yamashino T;Ishikawa A.
生物钟使植物能够适应其环境并控制许多生理过程，包括植物-病原体相互作用。然而，生物钟是否控制植物的非寄主抗性（NHR）尚不清楚。为了找到答案，我们用基于网络的工具DIURNAL分析了微阵列数据，揭示了在没有病原体挑战的情况下，NHR相关基因表现出有节奏的表达模式。我们的生物钟突变体分析发现，cca 1 -1 lhy-11双突变体对梨孢菌的NHR表现出受损，这表明生物钟的两个组成部分，CCA 1和LHY参与调节拟南芥的渗透抗性。通过对pen 2双突变体的分析，我们发现CCA 1作为一个正调节因子参与了一天中时间依赖的穿透抗性，而LHY作为一个正调节因子参与了穿透后抗性的调节。综上所述，我们的研究结果表明，昼夜节律钟调节一天中的时间依赖性NHR toP。oryzae并因此使A.缩写：EE：夜元素; ETI：效应子触发的免疫; NHR：非宿主抗性; PAMP：病原体相关分子模式; PTI：PAMP触发的免疫; SAR：系统获得性抗性。
The circadian clock enables plants to adapt to their environment and control numerous physiological processes, including plant–pathogen interactions. However, it is unknown if the circadian clock controls nonhost resistance (NHR) in plants. To find out, we analyzed microarray data with the web-based tool DIURNAL to reveal that NHR-related genes show rhythmic expression patterns in the absence of a pathogen challenge. Our clock mutant analyses found thatcca1-1 lhy-11double mutant showed compromised NHR toPyricularia oryzae, suggesting that two components of the circadian clock, CCA1 and LHY, are involved in regulating penetration resistance inArabidopsis thaliana. By analyzingpen2double mutants, we revealed that CCA1 contributes to time-of-day-dependent penetration resistance as a positive regulator and that LHY regulates post-penetration resistance as a positive regulator. Taken together, our results suggest that the circadian clock regulates the time-of-day-dependent NHR toP. oryzaeand thus enablesA. thalianato counteract pathogen attacks.Abbreviations:EE: evening element; ETI: effector-triggered immunity; NHR: nonhost resistance; PAMP: pathogen-associated molecular pattern; PTI: PAMP-triggered immunity; SAR: systemic acquired resistance.