Complete Nucleotide Sequence of Plasmids of Two Escherichia coli Strains Carrying blaNDM-5 and blaNDM-5 and blaOXA-181 From the Same Patient
Complete Nucleotide Sequence of Plasmids of Two Escherichia coli Strains Carrying blaNDM-5 and blaNDM-5 and blaOXA-181 From the Same Patient
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DOI：
10.3389/fmicb.2019.03095
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发表时间：
2020-01-21
影响因子：
5.2
通讯作者：
Migliavacca, Roberta
中科院分区：
文献类型：
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作者：
Marchetti, Vittoria Mattioni;Bitar, Ibrahim;Migliavacca, Roberta
本研究的目的是对两株产碳青霉烯酶的大肠埃希菌进行基因特征分析，这两株产碳青霉烯酶的大肠埃希菌均表达OXA-181和/或NDM-5型酶。上述微生物是在3年前意大利首次检测到bla(NDM-)(5)基因的同一家Desenzano医院(意大利北部)采集的。1株(5P)属于ST405/ST477序列型(按Pasture/Oxford方案)和O102：H6血清型。它的特征是含有一个130562 bp的多复制子载体IncFII/IncFIA/IncFIB(PVSI_NDM-5)，它包含两个主要的抗生素耐药岛：(I)ARI-I，大小10030 bp，携带β-内酰胺编码基因(blA(oxA-)(1)，bla(CTX-M-)(15))，氟喹诺酮/氨基糖苷(aac(6‘)-lb-cr)和苯尼考抗性(CatB3)，(Ii)ARI-II，大小15326 bp，携带磺胺(Sul1)编码基因，β-内酰胺(BLA(NDM-)(5)，BLA(TEM-)(1)(B))，苯尼考-(CatB3)，甲氧苄啶-(DfrA17)，防腐剂-(QacE Delta 1)，氨基糖苷-(aadA5，rmtB)耐药。另一株5m株，序列类型为ST2659/ST759，血清型为O50/02：H18型，携带4个质粒：153866个碱基的多复制子IncFII/IncFIA/IncFIB(pISV_IncFII_NDM-5)、89866个碱基的IncI1、51480个碱基的IncX3(PISVIncX3_OXA181)和41143个碱基的INCI(pISVInCi_CMY-42)。PISV_IncFII_NDM-5携带两个主要的抗生素耐药岛：(1)ARI-III，大小为12220个碱基，携带编码β-内酰胺(blA(oxA-)(1))、氟喹诺酮/氨基糖苷(aac(6‘)-lb-cr)、四环素(tet(B))和苯尼考抗性(catB3，catA1)的基因；和II)ARI-IV，大小为26527个碱基，携带编码大环内酯-(erm(B)，mph(A))，磺胺(Sul1)，β-内酰胺(BLA(NDM-)(5)，BLA(TEM-)(1)(B))，甲氧苄氨嘧啶(dfrA14，dfrA12)，防腐剂(QacE Delta 1)，氨基糖苷耐药(AadA5)。PISV_INCI_CMY-42含有β-内酰胺类耐药基因bla(CMY-)(42)，pISV_IncX3_OXA181含有氟喹诺酮类(QnrS1)和β-内酰胺类抗生素耐药基因bla(OXA-)(181)。总之，从一名有远东国家旅行史的儿科患者身上检测到两种不同的NDM-5大肠杆菌菌株，有力地突显了从这些地区输入的趋势和风险不断增加。
Aim of this study was to genetically characterize two carbapenemase-producing Escherichia coli strains obtained from a pediatric patient affected by diarrhea, expressing OXA-181 and/or NDM-5 type enzymes. The above microorganisms were collected in the same Desenzano hospital (Northern Italy) where the bla(NDM-)(5) gene was detected for the first time in Italy 3 years ago. One strain (5P), belonged to sequence type ST405/ST477 (according to Pasture/Oxford schemes) and serotype O102:H6. It was characterized by a 130562 bp multi-replicon plasmid IncFII/IncFIA/IncFIB (pVSI_NDM-5) enclosing two main antibiotic resistance islands: (i) ARI-I, 10030 bp in size, carried genes coding for beta-lactam- (bla(OXA-)(1), bla(CTX-M-)(15)), fluoroquinolone/aminoglycoside- (aac(6 ')-lb-cr) and phenicol- resistance (catB3), (ii) ARI-II, 15326 bp in size, carried genes coding for sulfonamide- (sul1), beta-lactam- (bla(NDM-)(5), bla(TEM-)(1)(B)), phenicol- (catB3), trimethoprim- (dfrA17), antiseptic- (qacE Delta 1), and aminoglycoside- (aadA5, rmtB) resistance. The other isolate (5M), belonged to sequence type ST2659/ST759 and serotype O50/02:H18, and carried four plasmids: a 153866 bp multi-replicon IncFII/IncFIA/IncFIB (pISV_IncFII_NDM-5), an 89866 bp IncI1 plasmid, a 51480 bp IncX3 plasmid (pISV_IncX3_OXA181), and a 41143 bp IncI plasmid (pISV_IncI_CMY-42). pISV_IncFII_NDM-5 carried two main antibiotic resistance islands: (i) ARI-III, 12220 bp in size, carried genes coding for beta-lactam- (bla(OXA-)(1)), fluoroquinolone/aminoglycoside- (aac(6 ')-lb-cr), tetracycline- (tet(B)) and phenicol- resistance (catB3, catA1), and ii) ARI-IV, 26527 bp in size, carried determinants coding for macrolide- (erm(B), mph(A)), sulfonamide- (sul1), beta-lactam- (bla(NDM-)(5), bla(TEM-)(1)(B)), trimethoprim- (dfrA14, dfrA12), antiseptic- (qacE Delta 1), and aminoglycoside- resistance (aadA5). pISV_IncI_CMY-42 harbored the bla(CMY-)(42) gene coding for beta-lactam resistance, pISV_IncX3_OXA181 harbored genes encoding fluoroquinolone- (qnrS1) and beta-lactams- resistance (bla(OXA-)(181)). In conclusion, the detection of two different NDM-5 E. coli strains from a pediatric patient with a history of travel to the Far East countries strongly highlight an increasing trend and risk of importation from such areas.