Bioorthogonal Microbubbles with Antifouling Nanofilm for Instant and Suspended Enrichment of Circulating Tumor Cells.
Bioorthogonal Microbubbles with Antifouling Nanofilm for Instant and Suspended Enrichment of Circulating Tumor Cells.
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DOI：
10.1021/acsnano.3c03194
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发表时间：
2023-05
期刊：
影响因子：
17.1
通讯作者：
Y-J Xiang;Hui Zhang;Hao Lu;Binqi Wei;Cuiyun Su;Xiaojie Qin;Minghong Fang;Xinchun Li;Fan Yang
中科院分区：
文献类型：
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作者：
Y-J Xiang;Hui Zhang;Hao Lu;Binqi Wei;Cuiyun Su;Xiaojie Qin;Minghong Fang;Xinchun Li;Fan Yang
整合临床稀有细胞富集、培养和单细胞表型分析目前受到缺乏胜任技术的阻碍，这些技术通常遭受弱细胞界面碰撞亲和力、强非特异性吸附和潜在摄取。在这里，我们报告了Cells-on-a-bubble，一种生物启发的，自供电的生物正交微泡（点击气泡），它利用可点击的纳米界面和DNA组装的吸盘样多价细胞表面，能够在几分钟内即时和悬浮分离循环肿瘤细胞（CTC）。使用这种仿生工程策略，点击气泡的捕获效率高达98%，比单价对应物快15倍，提高了20%。此外，浮力激活的气泡促进捕获的单个癌细胞的自分离、3D悬浮培养和原位表型分析。通过使用多抗体设计，这种快速、经济实惠的微型马达样点击气泡能够在三种癌症类型的队列（n = 42）中悬浮富集CTC，并进行治疗反应评估，这意味着其在单细胞分析和3D类器官培养方面具有巨大潜力。
Integrating clinical rare cell enrichment, culture, and single-cell phenotypic profiling is currently hampered by the lack of competent technologies, which typically suffer from weak cell-interface collision affinity, strong nonspecific adsorption, and the potential uptake. Here, we report cells-on-a-bubble, a bioinspired, self-powered bioorthogonal microbubble (click bubble) that leverages a clickable antifouling nanointerface and a DNA-assembled sucker-like polyvalent cell surface, to enable instant and suspended isolation of circulating tumor cells (CTCs) within minutes. Using this biomimetic engineering strategy, click bubbles achieve a capture efficiency of up to 98%, improved by 20% at 15 times faster over their monovalent counterparts. Further, the buoyancy-activated bubble facilitates self-separation, 3D suspension culture, and in situ phenotyping of the captured single cancer cells. By using a multiantibody design, this fast, affordable micromotor-like click bubble enables suspended enrichment of CTCs in a cohort (n = 42) across three cancer types and treatment response evaluation, signifying its great potential to enable single-cell analysis and 3D organoid culture.