Application of cationic polymer micelles for the dispersal of bacterial biofilms
Application of cationic polymer micelles for the dispersal of bacterial biofilms
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DOI：
10.1002/elsc.201800040
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发表时间：
2018-12-01
影响因子：
2.7
通讯作者：
Paunova-Krasteva, Tsvetelina
中科院分区：
文献类型：
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作者：
Borisova, Dayana;Haladjova, Emi;Paunova-Krasteva, Tsvetelina
医院和食品工业的表面被细菌生物膜污染是一种严重的健康风险。值得关注的是它们对常规抗菌剂和消毒剂的耐药性。这需要开发新的生物膜分离方法。该研究评估了阳离子聚合物胶束（CPM）对预形成的生物膜的有效性。使用基于不同聚阳离子的CPM。颗粒的流体动力学半径范围为16至360 nm。将大肠杆菌420、铜绿假单胞菌PAO 1、金黄色葡萄球菌29213和枯草芽孢杆菌168的生物膜培养24 h，然后用CPM处理预形成的生物膜2、4或6 h。通过结晶紫测定法评价生物膜生物量，并应用活/死荧光试验测定细菌活力。评价了CPM与模型菌株的预形成生物膜相互作用的能力。我们观察到，最有效的CPM是那些基于聚（2-（二甲基氨基）乙基甲基丙烯酸酯）共聚物，减少生物膜生物量的三至四倍相比，与水处理的生物膜。通过活/死染色记录生物膜中细菌的活力显著降低。结果表明CPM用于生物膜污染表面消毒和伤口治疗的适用性。
Contamination of surfaces in hospitals and food industry by bacterial biofilms is a serious health risk. Of concern is their resistance to routine antibacterials and disinfectants. This requires the development of novel approaches to biofilm detachment. The study evaluates the effectiveness of cationic polymer micelles (CPMs) against pre-formed biofilms. CPMs based on different polycations were used. The hydrodynamic radius of the particles ranged from 16 to 360 nm. Biofilms of Escherichia coli 420, Pseudomonas aeruginosa PAO1, Staphylococcus aureus 29213 and Bacillus subtilis 168 were cultivated for 24 h then the pre-formed biofilms were treated with the CPMs for 2, 4 or 6 h. Biofilm biomass was evaluated by the crystal violet assay, and live/dead fluorescence test was applied for bacterial viability. The ability of CPMs to interact with pre-formed biofilms of the model strains was evaluated. We observed that the most effective CPMs were those based on poly(2-(dimethylamino)ethyl methacrylate) copolymers which reduced the biofilm biomass three- to four-fold compared with the treatment of the biofilm with water. Significantly reduced vitality of the bacteria in the biofilms was registered by the live/dead stain. The results indicate the applicability of the CPMs for disinfection of biofilm-contaminated surfaces and the treatment of wounds.