COMPARATIVE GENOME ANALYSIS OF MUNGBEAN (VIGNA-RADIATA L-WILCZEK) AND COWPEA (V-UNGUICULATA L WALPERS) USING RFLP MAPPING DATA
COMPARATIVE GENOME ANALYSIS OF MUNGBEAN (VIGNA-RADIATA L-WILCZEK) AND COWPEA (V-UNGUICULATA L WALPERS) USING RFLP MAPPING DATA
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DOI：
10.1007/bf00212605
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发表时间：
1993-08-01
影响因子：
5.4
通讯作者：
YOUNG, ND
中科院分区：
文献类型：
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作者：
MENANCIOHAUTEA, D;FATOKUN, CA;YOUNG, ND
基于随机基因组限制性片段长度多态性（RFLP）标记的连锁排列，研究了绿豆（Vigna radiata）和豇豆（V. unguiculata）的基因组关系。利用来自豇豆、普通豆（Phaseolus vulgaris）、绿豆和大豆（Glycine max）基因组文库的一组探针构建遗传连锁图谱。在这两个物种中，利用一个改良品种与一个假定的野生祖先种杂交的F2群体来跟踪RFLP标记的分离。大约90%的探针与绿豆和豇豆DNA杂交，表明这些物种之间的核苷酸序列高度相似。绿豆群体的多态性水平（75.7%）高于豇豆群体（41.2%）。在两个种群中均检测到重复、零表型和扭曲分离率的基因座。随机基因组DNA RFLP位点约占目前定位标记的89%，并添加了少量cDNA和RAPD标记。目前的绿豆图谱由171个位点/位点簇组成，分布在14个连锁群中，全长1570 cM。另一方面，在目前的豇豆图谱中，97个标记覆盖了684 cM，定义了10个连锁群。根据绿豆和豇豆共有的53个标记的拷贝数和连锁排列，比较了绿豆和豇豆的基因组。结果表明，这两个物种的核苷酸序列是保守的，但拷贝数发生了变化，并且在连锁顺序上发生了多次重排。整个连锁组不保守，但在两个基因组中都保持了几个大的连锁块。
Genome relationships between mungbean (Vigna radiata) and cowpea (V. unguiculata) based on the linkage arrangement of random genomic restriction fragment length polymorphism (RFLP) markers have been investigated. A common set of probes derived from cowpea, common bean (Phaseolus vulgaris), mungbean, and soybean (Glycine max) PstI genomic libraries were used to construct the genetic linkage maps. In both species, a single F2 population from a cross between an improved cultivar and a putative wild progenitor species was used to follow the segregation of the RFLP markers. Approximately 90% of the probes hybridized to both mungbean and cowpea DNA, indicating a high degree of similarity in the nucleotide sequences among these species. A higher level of polymorphism was detected in the mungbean population (75.7%) than in the cowpea population (41.2%). Loci exhibiting duplications, null phenotypes, and distorted segregation ratios were detected in both populations. Random genomic DNA RFLP loci account for about 89% of the currently mapped markers with a few cDNA and RAPD markers added. The current mungbean map is comprised of 171 loci/loci clusters distributed in 14 linkage groups spanning a total of 1570 cM. On the other hand, 97 markers covered 684 cM and defined 10 linkage groups in the current cowpea map. The mungbean and cowpea genomes were compared on the basis of the copy number and linkage arrangement of 53 markers mapped in common between the two species. Results indicate that nucleotide sequences are conserved, but variation in copy number were detected and several rearrangements in linkage orders appeared to have occurred since the divergence of the two species. Entire linkage groups were not conserved, but several large linkage blocks were maintained in both genomes.