Amino-functionalized green fluorescent carbon dots as surface energy transfer biosensors for hyaluronidase
Amino-functionalized green fluorescent carbon dots as surface energy transfer biosensors for hyaluronidase
复制标题
氨基功能化的绿色荧光碳点作为透明质酸酶的表面能量转移生物传感器。
DOI：
10.1039/c5nr00070j
复制
发表时间：
2015-01-01
期刊：
影响因子：
6.7
通讯作者：
Liu, Hongguang
中科院分区：
文献类型：
--
作者：
Liu, Siyu;Zhao, Ning;Liu, Hongguang
氨基功能化的荧光碳点已通过水热处理葡萄糖胺与过量的焦磷酸盐制备。所制备的碳量子点在不同的激发波长和pH环境下显示稳定的绿色发射荧光。在此，我们证明了氨基官能化的碳量子点和带负电荷的透明质酸盐稳定的金纳米颗粒之间的表面能量转移。透明质酸酶可以降解透明质酸并分解透明质酸稳定的金纳米颗粒以抑制表面能量转移。所开发的荧光碳点/金纳米颗粒系统可以用作生物传感器，通过两种模式，包括荧光测量和比色分析的透明质酸酶的敏感和选择性的检测。
Amino-functionalized fluorescent carbon dots have been prepared by hydrothermal treatment of glucosamine with excess pyrophosphate. The produced carbon dots showed stabilized green emission fluorescence at various excitation wavelengths and pH environments. Herein, we demonstrate the surface energy transfer between the amino-functionalized carbon dots and negatively charged hyaluronate stabilized gold nanoparticles. Hyaluronidase can degrade hyaluronate and break down the hyaluronate stabilized gold nanoparticles to inhibit the surface energy transfer. The developed fluorescent carbon dot/gold nanoparticle system can be utilized as a biosensor for sensitive and selective detection of hyaluronidase by two modes which include fluorescence measurements and colorimetric analysis.