Cloning of a human tRNA isopentenyl transferase
Cloning of a human tRNA isopentenyl transferase
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DOI：
10.1016/s0378-1119(00)00421-2
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发表时间：
2000-11-27
期刊：
影响因子：
3.5
通讯作者：
Nicander, B
中科院分区：
文献类型：
--
作者：
Golovko, A;Hjälm, G;Nicander, B
人来源的cDNA显示编码tRNA异戊烯基转移酶（E.C. 2.5.1.8）。在缺乏内源性tRNA异戊烯基转移酶MOD 5的酿酒酵母突变体中表达该基因导致功能互补和将异戊烯基腺苷重新引入tRNA。推导的氨基酸序列包含已知tRNA异戊烯基转移酶中保守的许多区域。与S.酵母MOD5蛋白的表达率为53%，大肠杆菌MiaA蛋白的表达率为47%。人的序列被发现含有一个单一的C2H2锌指样基序，这也被检测到的MOD 5蛋白，和几个假定的tRNA转移酶位于BLAST搜索，但不是在原核同源物。(C)2000爱思唯尔科技有限公司。保留所有权利。
A cDNA of human origin is shown to encode a tRNA isopentenyl transferase (E.C. 2.5.1.8). Expression of the gene in a Saccharomyces cerevisiae mutant lacking the endogenous tRNA isopentenyl transferase MOD5 resulted in functional complementation and reintroduction of isopentenyladenosine into tRNA. The deduced amino acid sequence contains a number of regions conserved in known tRNA isopentenyl transferases. The similarity to the S. cerevisiae MOD5 protein is 53%, and to the Escherichia coli MiaA protein 47%. The human sequence was found to contain a single C2H2 Zn-finger-like motif, which was detected also in the MOD5 protein, and several putative tRNA transferases located by BLAST searches, but not in prokaryotic homologues. (C) 2000 Elsevier Science B.V. All rights reserved.